Severe Sepsis Septic shock Chronic critical illness Immunosuppression Healthy volunteer A41 G62.8 G72.8
Conditions
Interventions
Group 1: Isolation of peripheral T cells and investigation of their functional status: proliferation and activation markers via FACS analysis, signal transduction via biochemical and molecular biologi
Sponsors
Integriertes Forschungs- und Behandlungszentrum
Center of Sepsis Control and Care
Paul-Martini-Forschergruppe ”Clinical Septomics”
Eligibility
Sex/Gender
Male
Age
55 Years to 80 Years
Inclusion criteria
Inclusion criteria: 1. Male patients, age 55-80 years 2. State after SIRS / sepsis with multiple organ failure in the pre-treating acute hospital 3. Presence of critical illness polyneuropathy and myopathy (CIPNP and CIMP) 4. Chronic critical illness with 2-organ failure: - Prolonged mechanical ventilation > 3 weeks and < 12 weeks - state after acute renal failure and currently running renal replacement therapy
Exclusion criteria
Exclusion criteria: 1. Previous chemotherapy or radiation therapy 2. C-reactive protein mg> 140 / L 3. Chronic immunosuppression 4. Treatment with Prednisolone within the last 8 days before taking blood samples 5. Current septic inflammation 6. Chronic renal failure with ambulant dialysis 7. Current therapy with vasopressors 8. Acute Lung Injury (ALI): FiO2> 30% 9. Traumatic Brain Injury 10. Intracerebral hemorrhage / stroke within the last 12 weeks
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Assessment of the functionality of T-cells by means of measuring biochemical and cellbiological parameters of activation: Proliferation / activation markers via FACS analysis Signaltransduction analysis via biochemical assays | — |
Secondary
| Measure | Time frame |
|---|---|
| - Causal link between the results of the immunological studies with metabolically-malnutritiven and neuroendocrine changes in patients - Sepsis history in acute hospital (focus, microbiology, nature, severity and duration of organ failure, surgical interventions) - Polyneuropathy / myopathy - Scores: APACHE II score, NRS 2002 - Duration of mechanical ventilation (days), renal replacement therapy (days), hospital length of stay (days), enteral / parenteral nutrition (kcal / kg / day) | — |
Countries
Germany
Contacts
Public ContactFrank Martin Brunkhorst
Integriertes Forschungs- und Behandlungszentrum Center of Sepsis Control and Care Paul-Martini-Forschergruppe ”Clinical Septomics”
Outcome results
None listed