Pathogen associated inflammation and impaired spermatogenesis in TESE samples form patients with azoospermia

Pathogen associated inflammation and impaired spermatogenesis in TESE samples form patients with azoospermia - LOEWE MIBIE B1

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

DRKS

Registry ID

DRKS00003481

Enrollment

100

Registered

2012-01-10

Start date

2011-05-12

Completion date

Unknown

Last updated

2025-04-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

N46

Interventions

Group 1: patients with non-obstructive azoospermia and TESE will recieve the following investigations: Medical histody, clinical investigation, urinanalysis, blood investigation (sexual hormones), sem

Eligibility

Sex/Gender

Male

Age

18 Years to No maximum

Inclusion criteria

Inclusion criteria: Azoospermia with infertility and request for therapy

Exclusion criteria

Exclusion criteria: palpable testicular cancer

Design outcomes

Primary

Measure	Time frame
Investigation of the testicular Inflammation (immune cells, cytokines, pathogens) with respect to impaired spermatogenesis (histologic score according to Bergmann and Kliesch, expression of protamines) in testicular tissue (TESE samples) of males with azoospermia	—

Secondary

Measure	Time frame
• determine the testicular sperm retrieval rate using TESE • investigate vascular changes in testicular histology (atherosclerosis) • determine the prevalence of hypogonadism before and after TESE surgery • determine differences between patients with HIV and hepatitis C • investigation of the hormonal status (FSH, LH, inhibin, testesterone, oestradiol, prolactin) and correlation with spermatogenesis • determine inflammatory parameters in the male genitourinary tract (2 resp. 4-glas test, leukocytes in urine, PSA in blood, inflammatory parameters in semen: leukocytes, elastase, cytokines) • determine comorbidities (anamnesis, clinical investigation, laboratory parameters, medical reports) • determine ultrasonographic features of male sex organs (testicular size, epididymal size, perfusion of the spermatic cord, testis, and epididymis, size and echogenity of the prostate) • establish a functional MRI of the testis • determine the impact of infertility on quality of life using questionnaires (SF-12, HADS-D, HRS) • investigate the impact of infertility on sexual function with standardized interview and questionnaires (NIH-CPSI, IIEF, HRS) • investigate genetic risk factors associated with azoospermia (Klinfelter syndrome, CTFR-mutations, AZF-deletions) • investigate the prevalence of sexual transmitted diseases (Chlamydia, Mycoplasma, Gonorrhoeae) • determine the benefit of 16 S rRNA gene sequencing for the detection of pathogens in urine and semen • compare the outcomes of patients with obstructive and non-obstructive azoospermia.	—

Measure

Time frame

• determine the testicular sperm retrieval rate using TESE • investigate vascular changes in testicular histology (atherosclerosis) • determine the prevalence of hypogonadism before and after TESE surgery • determine differences between patients with HIV and hepatitis C • investigation of the hormonal status (FSH, LH, inhibin, testesterone, oestradiol, prolactin) and correlation with spermatogenesis • determine inflammatory parameters in the male genitourinary tract (2 resp. 4-glas test, leukocytes in urine, PSA in blood, inflammatory parameters in semen: leukocytes, elastase, cytokines) • determine comorbidities (anamnesis, clinical investigation, laboratory parameters, medical reports) • determine ultrasonographic features of male sex organs (testicular size, epididymal size, perfusion of the spermatic cord, testis, and epididymis, size and echogenity of the prostate) • establish a functional MRI of the testis • determine the impact of infertility on quality of life using questionnaires (SF-12, HADS-D, HRS) • investigate the impact of infertility on sexual function with standardized interview and questionnaires (NIH-CPSI, IIEF, HRS) • investigate genetic risk factors associated with azoospermia (Klinfelter syndrome, CTFR-mutations, AZF-deletions) • investigate the prevalence of sexual transmitted diseases (Chlamydia, Mycoplasma, Gonorrhoeae) • determine the benefit of 16 S rRNA gene sequencing for the detection of pathogens in urine and semen • compare the outcomes of patients with obstructive and non-obstructive azoospermia.

—

Countries

Germany

Contacts

Public ContactThorsten Diemer

Klinik und Poliklinik für Urologie, Kinderurologie und Andrologie, Universitätsklinikum Gießen und Marburg GmbH, Standort Gießen, Justus-Liebig-Universität Gießen

thorsten.diemer@chiru.med.uni-giessen.de0641-985-44505

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Feb 4, 2026