A31 I88
Conditions
Interventions
Group 1: children with NTM Lymphadenitis
Group 2: controls matched for sex, age, region
Sponsors
Universitätsklinikum Freiburg Institut für Immundefizienz
Eligibility
Sex/Gender
All
Age
No minimum to 18 Years
Inclusion criteria
Inclusion criteria: Children with NTM infection defined as follows: 1) Probable infection Unilaterarally enlarged lymph node >= 2cm in diameter plus TST skin test > 5 mm plus exclusion of tuberculosis/ malignant lymphoma/ bartonellosis 2) Confirmed NTM infection a) Lymph node biopsy with histology typical for mycobacteria plus positive culture or PCR for NTM or b) Draining lymphnode plus positive smear (culture or PCR) for NTM Controls will be matched by age, sex, region, and – if possible – by ethnicity. The second unmatched control group will consist of children with diagnosed Bartonella henselae infection (specific antibody (IgG) titre > 1:265 in the course of the disease).
Exclusion criteria
Exclusion criteria: 1) Lack of informed consent of parent(s) or lack of assent of patient 2) Malignom/lymphoma, lymphoproliferative disease, detection of other pathogens (particularly Bartonella henselae (by culture, PCR or serology), tuberculosis (IGRA, Ellispot or x-ray chest for TB exclusion), detection of acute infections with other pathogens by culture, PCR or serology (Toxoplasmosis, infections with actinomycetes, Nocardia, CMV, EBV, HIV). 3) Children hospitalized due to immune or infection related conditions will not be eligible as controls for this study
Design outcomes
Secondary
| Measure | Time frame |
|---|---|
| Questionnaire data: Lifestyle and environmental risk factors for NTM infections Genetics: Frequency of genetic variants in candidate genes between NTM cases and controls Micronutrients: Differences in immuno-nutrients (zinc, selenium etc.) between NTM cases and controls (Determination through questionnaire data and blood sample and buccal swap taken at study entrance). | — |
Primary
| Measure | Time frame |
|---|---|
| Primary Target Variable Micronutrients: Differences in serum 25(OH)vitamin D2 and D3 levels between NTM-cases and controls DNA-Methylation: Differences in DNA-Methylation levels of selected antimycobacterial candidate genes between NTM-cases and controls (Determination through blood sample and buccal swap taken at study entrance). | — |
Countries
Germany
Contacts
Public ContactPhilipp Henneke
Universitätsklinikum Freiburg Institut für Immundefizienz
Outcome results
None listed