Infections caused by Klebsiella pneumoniae, including pneumonia, bacteremia, liver abscess, urinary tract infection
Conditions
Interventions
ESBL-producing multidrug-resistant group:None
Carbapenem-resistant Klebsiella pneumoniae (CRKP) group:None
Hypervirulent Klebsiella pneumoniae (hvKP) group:None
Sponsors
The First Affiliated Hospital of the Baotou Medical College of Inner Mongolia University of Science and Technology
Eligibility
Sex/Gender
All
Age
18 Years to 80 Years
Inclusion criteria
Inclusion criteria: 1. Strains are identified as Klebsiella pneumoniae by VITEK mass spectrometry or 16S rRNA sequencing; 2. Clear origin: clinical isolates (specimens from pulmonary infection, bloodstream infection, liver abscess and urinary tract) or standard reference strains (ATCC 700603, hvKP ATCC BAA-2146, carbapenem-resistant CRKP strains); 3. After resuscitation, strains exhibit favorable growth on plates without microbial contamination and show stable subculture performance; 4. Clinical isolates are accompanied by complete medical record information, including infection site, antibiotic medication history, antimicrobial susceptibility results and patients' underlying diseases, which supports grouping (cKP/hvKP/CRKP); 5. Strains can be stably cryopreserved and repeatedly passaged, without spontaneous degradation or plasmid loss.
Exclusion criteria
Exclusion criteria: 1. Strains identified as other Klebsiella species by mass spectrometry or gene sequencing (including Klebsiella oxytoca, Klebsiella rhinoscleromatis, etc.); 2. Mixed contaminating bacteria observed via plate microscopy; 1.single colonies cannot be obtained after repeated purification; 3. Strains exhibit degeneration, retarded growth and spontaneous autolysis after multiple passages, which prevents RNA extraction and phenotypic experiments; 4. Clinical isolates without complete medical records or antimicrobial susceptibility data, making grouping analysis unavailable; 5. Strains carry lethal defects and display abnormal cell division, failing to support the construction of gene knockout/overexpression strains; 6. Strains degraded due to improper preservation and repeated freeze-thaw cycles, resulting in poor RNA integrity (RIN < 6.0).
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Relative mRNA expression of target gene; | — |
Secondary
| Measure | Time frame |
|---|---|
| Age; MIC; Site of infection; | — |
Countries
China
Contacts
Public ContactLiu Ying
The First Affiliated Hospital of the Baotou Medical College of Inner Mongolia University of Science and Technology
Outcome results
None listed