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Investigating the Effects and Underlying Mechanisms of Hot Rule Whey Protein Complex (Mass Gainer) on Weight and Muscle Gain across Diverse Populations

Investigating the Effects and Underlying Mechanisms of Hot Rule Whey Protein Complex (Mass Gainer) on Weight and Muscle Gain across Diverse Populations

Status
Active, not recruiting
Phases
Unknown
Study type
Interventional
Source
ChiCTR
Registry ID
ChiCTR2600129185
Enrollment
Unknown
Registered
2026-07-31
Start date
2026-08-01
Completion date
Unknown
Last updated
2026-08-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None

Interventions

Placebo group(study one):Take 45 g of isomaltooligosaccharide and maltodextrin before 9:00 a.m., and another 45 g before bedtime, each dissolved in 250 mL of warm water.
Hot Rule Whey Protein Complex Powder Supplementation Group (Study 1):Take 45 g of Hot Rule whey protein complex powder before 9:00 a.m., and another 45 g before bedtime, each dissolved in 250 mL of wa
Resistance training group + Placebo group(study two):Take 45 g of isomaltooligosaccharide and maltodextrin after resistance exercise, and another 45 g before bedtime, each dissolved in 250 mL of warm
Resistance training + Hot Rule whey protein supplement group(study two):Take 45 g of Hot Rule whey protein complex powder after resistance exercise, and another 45 g before bedtime, each dissolved in
Glucose group (Study three):Take 45 g of anhydrous glucose, dissolved in 250 mL of warm water.
Hot Rule Whey protein complex powder supplementation group(study three):Take 45 g of Hot Rule whey protein complex powder, dissolved in 250 mL of warm water.(One week after the test for the glucose gr

Sponsors

Shanghai University of Sport
Lead Sponsor

Eligibility

Sex/Gender
Male
Age
18 Years to 35 Years

Inclusion criteria

Inclusion criteria: Inclusion criteria for Study one: 1. Priority is given to individuals with BMI = 3 times/week). 2. No use of protein powder, mass gainers, vitamins, other nutritional supplements, or hormonal medications in the three months prior to the start of the study. Inclusion criteria for Study three: 1. Normal BMI range: 18.5–24. 2. Young males aged 18–35 who had no regular exercise in the six months prior to the study (<= 2 times/week). 3. No use of protein powder, mass gainers, vitamins, other nutritional supplements, or hormonal medications in the three months prior to the start of the study.

Exclusion criteria

Exclusion criteria: Exclusion criteria for Study one : Individuals with impaired or severely compromised liver or kidney function; Those with diabetes, gout, or hyperuricemia; Individuals allergic to the ingredients in mass gainers, or strict vegetarians (vegans). Exclusion criteria for Study two: Individuals with impaired or severely compromised liver or kidney function; Those with diabetes, gout, or hyperuricemia; Individuals allergic to the ingredients in mass gainers, or strict vegetarians (vegans); Individuals with clear contraindications to exercise or other conditions that may affect participation in an exercise program. Exclusion criteria for Study three : Individuals with impaired or severely compromised liver or kidney function; Those with diabetes, gout, or hyperuricemia; Individuals allergic to the ingredients in mass gainers, or strict vegetarians (vegans).

Design outcomes

Primary

MeasureTime frame
Body composition (total muscle mass, total fat mass, visceral fat content, subcutaneous fat content, body fat percentage, muscle/fat mass in upper/lower limbs/torso, bone mineral density);Maximal upper limb muscle strength (bench press 1RM);weight;Body measurements (upper arm, shoulder, chest, waist, hip, thigh, calf);Upper limb muscle endurance;Lower limb muscle endurance;Maximum lower limb muscle strength (vertical jump);Upper limb motor performance (medicine ball throwing);Lower limb motor performance (30m sprint run);Blood tests (fasting blood glucose);Maximum muscle strength of the upper limb (grasp force);

Secondary

MeasureTime frame
height;Blood parameters (creatinine, blood urea nitrogen, uric acid, testosterone, cortisol);Bowel reactions (Bowel tolerance questionnaire);

Countries

China

Contacts

Public ContactXiao Weihua

Shanghai University of Sport

xiao_weihua@163.com+86 158 2129 9145

Outcome results

None listed

Source: ChiCTR (via WHO ICTRP) · Data processed: Aug 10, 2026