Second-degree burns
Conditions
Interventions
Negative pressure treatment group after scab ablation:The patient first underwent scab ablation (using a scab grinder to remove necrotic tissue from the wound while preserving the healthy dermis), and
The patient first underwent scab ablation (using a scab grinder to remove necrotic tissue from the wound while preserving the healthy dermis), and immediately after the operation, negative pressure cl
Sponsors
Affiliated Hospital of Jiangnan University
Eligibility
Sex/Gender
All
Age
18 Years to 65 Years
Inclusion criteria
Inclusion criteria: 1. Aged 18–65 years, no gender restrictions. 2. Confirmed deep second-degree burns covering 5–20% of total body surface area, with 20 subjects per group. 3. Time from injury to admission <=72 hours, with no prior specialised wound treatment (e.g., skin grafting, growth factor application). 4. No severe dysfunction of vital organs (heart, liver, kidneys, etc.), no coagulation disorders, no malignant tumours, and no immunodeficiency diseases. 5. Informed consent obtained from both patient and family members, with signed consent forms.
Exclusion criteria
Exclusion criteria: 1. The wound is complicated with severe infection (such as excessive purulent discharge from the wound, obvious local redness, swelling, heat and pain, and blood routine shows a white blood cell count >15×10^9/L and a neutrophil ratio >85%). 2. People who are allergic to negative pressure treatment materials. 3. Pregnant or lactating women. 4. Those with poor compliance and unable to cooperate to complete the entire treatment cycle and sample collection.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Inflammatory indicators of the drainage fluid (tumor necrosis factor -a (TNF-a), interleukin-6 (IL-6), interleukin-10 (IL-10).); | — |
Secondary
| Measure | Time frame |
|---|---|
| Growth factor indicators (vascular endothelial growth factor (VEGF), fibroblast growth factor (bFGF));Matrix metalloproteinases MMPs (MMP-2, MMP-9);Metabolic indicators (total protein, glucose, lactate dehydrogenase, adenosine deaminase);The drainage fluid precipitates the RNA of cells;The effects of VSD drainage fluid on the proliferation, apoptosis and function of fibroblasts and keratinocytes cultured in vitro;Adverse event; | — |
Countries
China
Contacts
Public ContactCheng Jia
Affiliated Hospital of Jiangnan University
Outcome results
None listed