Perceived stress and mental status
Conditions
Interventions
Probiotic group:Bifidobacterium longum subspecies combination (dipro-O + dipro-X), 5×10^9 CFU per bag, once daily, 1 bag each time, oral administration, for 8 consecutive weeks
Placebo group:Placebo (erythritol, xylitol, blueberry juice powder, etc.), 0 CFU per bag, once daily, 1 bag each time, orally, for 8 consecutive weeks
Sponsors
Dalian Medical University
Eligibility
Sex/Gender
All
Age
18 Years to 65 Years
Inclusion criteria
Inclusion criteria: 1. Aged 18–65 years; 2. PSS-10 score >= 14; 3. Voluntarily participate in this study and sign written informed consent before any study procedures begin.
Exclusion criteria
Exclusion criteria: 1. Use of probiotic supplements or probiotic-containing products within 4 weeks before enrollment; 2. Use of antibiotics or systemic glucocorticoids within 4 weeks before enrollment; 3. Current or previous diagnosis of psychiatric disorders (e.g., schizophrenia, epilepsy, major depressive disorder, etc.), major endocrine system diseases, active inflammatory bowel disease or other severe gastrointestinal disorders, or severe sleep disturbances requiring treatment adjustment; 4. Use of medications or dietary supplements that may affect the brain-gut axis, sleep, mood, or stress status assessment within 4 weeks before enrollment, including but not limited to sedative-hypnotics, anxiolytics, antidepressants, antipsychotics, mood stabilizers, and supplements with sleep-inducing, sedative, or stress-relieving effects (e.g., magnesium-containing supplements); 5. Pregnant or breastfeeding women; 6. Known allergy to the study product or any of its components; 7. Smoking or alcohol consumption on the day of the visit or within 48 hours before the visit; 8. Participation in other clinical intervention studies on drugs, dietary supplements, probiotics, or prebiotics within the past 3 months; 9. Insufficient compliance as judged by the investigator to complete sample collection or study-related documentation as required by the study protocol.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Stress status score; | — |
Secondary
| Measure | Time frame |
|---|---|
| Sleep quality score;Quality of life score;Gut microbiota;Gut stress biomarkers;Gut immune markers; | — |
Countries
China
Contacts
Public ContactLi Huajun
Dalian Medical University
Outcome results
None listed