Oral Mucosal Diseases
Conditions
Interventions
Experimental group:Limited consumption of areca nuts (3 nuts) for a short period (within 3 hours)
Sponsors
Xiangya Stomatological Hospital, Central South University,Hunan
Eligibility
Sex/Gender
Male
Age
20 Years to 40 Years
Inclusion criteria
Inclusion criteria: 1. Age 20-40 years, male; 2. No regular betel nut chewing habit (defined as: in the past six months, the average frequency of betel nut chewing is less than once per month, and the total number of times does not exceed 10, with no betel nut chewing in the 1 month prior to enrollment); 3. Confirmed by oral examination to have no oral mucosal lesions (such as leukoplakia, erythroplakia, erosion, ulcers, etc.) and no related medical history; 4. No smoking habit (defined as: never smoked, or previously smoked occasionally but has quit for >= 6 months); 5. Good compliance, voluntarily participating in this study, able to understand the study procedures, and willing to sign a written informed consent form.
Exclusion criteria
Exclusion criteria: 1. Patients with any systemic diseases, such as: coronary heart disease, hypertension and other cardiovascular diseases, cerebral infarction and other cerebrovascular diseases, chronic obstructive pulmonary disease and other lung diseases, gastritis and gastroduodenal ulcer and other gastrointestinal diseases, chronic hepatitis and cirrhosis liver diseases, chronic nephritis and other renal dysfunction or diseases, rheumatoid arthritis and systemic lupus erythematosus and other immune system diseases, diabetes, hyperlipidemia, osteoporosis, cancer, moderate to severe illnesses with or without fever within 3 months, serious mental illnesses, etc.; 2. Within 1 month before sampling: the person has used any antibiotics, antifungal, antiviral, or antiparasitic drugs (oral, nebulized, intramuscular, or intravenous), used any probiotic products (tablets, capsules, lozenges, powders, etc.), or received any immunotherapy, radiotherapy, or chemotherapy; 3. The following situations may occur during oral health screening: (1) Oral mucosa with sensations such as roughness, itching, crawling sensation, dry mouth, loss of taste, numbness of lips and tongue; (2) Periodontal pocket > = 4 mm; (3) More than 10% of sites have post-probing bleeding; (4) Untreated caries or abscess; (5) Precancerous lesions or evidence of cancerous transformation; (6) Candida infection; (7) Clinically significant halitosis; (8) Missing more than 4 teeth in addition to the third molar (including loss due to trauma, orthodontic extraction needs, or congenital tooth loss); (9) Have undergone local oral trauma surgery, received periodontal treatment within the past 3 months, or are currently undergoing orthodontic treatment; 4. History of viral infection, influenza, or other infectious diseases within 1 month prior to sampling; 5. History of betel nut or other food allergies; 6. Use of any tobacco products within 1 month prior to sampling; 7. HIV carriers; 8. Individuals deemed unsuitable for the trial by the researcher for other reasons.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Alterations Oral Microbiota Beta Diversity Before and After Areca Nut Chewing;Changes in the Oral/Salivary Metabolome Before and After Areca Nut Chewing; | — |
Secondary
| Measure | Time frame |
|---|---|
| Change in Heart Rate;Change in Systolic Blood Pressure;Change in Diastolic Blood Pressure;Change in Respiratory Rate;Change in Body Temperature;Change in Unstimulated Whole Saliva Flow Rate; | — |
Countries
China
Contacts
Public ContactOusheng Liu
Xiangya Stomatological Hospital & Xiangya School of Stomatology, Central South University, Hunan
Outcome results
None listed