intestinal barrier injury
Conditions
Interventions
Gold Standard:Dual sugar test (lactulose/mannitol ratio)
Index test:Diagnostic model for intestinal barrier injury based on metabolomic profiling
Sponsors
General Hospital of the Eastern Theater Command of the People's Liberation Army of China (Jinling Hospital Affiliated to Nanjing University Medical School)
Eligibility
Sex/Gender
All
Age
18 Years to 80 Years
Inclusion criteria
Inclusion criteria: Critically Ill Patients: 1. Age 18 to 80 years; 2. APACHE II score = 12; 3. No history of gastrointestinal diseases or surgery; 4. Provision of informed consent for sample collection. Patients with Inflammatory Bowel Disease: 1. Age 18 to 80 years; 2. No infectious diseases within the past month; 3. Meeting the clinical diagnostic criteria for ulcerative colitis (UC) or Crohn's disease (CD) (confirmed by radiological, endoscopic, or pathological examination); 4. No history of other gastrointestinal diseases or surgery; 5. Provision of informed consent for sample collection. Healthy Controls: 1. Age 18 to 80 years, in good general health; 2. No infectious diseases within the past month; 3.No history of gastrointestinal diseases or surgery; 4. Provision of informed consent for sample collection. Inclusion criteria for abdominal surgery patients: 1. Age 18 to 80 years; 2. Patients undergoing elective abdominal surgery; 3. Agree to sample collection and have the corresponding informed consent form.
Exclusion criteria
Exclusion criteria: 1. Pregnant or lactating women; 2. Patients with concomitant hepatic or renal dysfunction; 3. Patients with concomitant endocrine and metabolic diseases such as hypertension, diabetes, thyroid disorders, or other immune-related diseases; 4. Alcohol consumption or use of non-steroidal anti-inflammatory drugs, immunosuppressants, antimicrobial agents, probiotics, hormonal medications, gastric acid secretion inhibitors, or other drugs that may affect intestinal permeability within 2 weeks prior to enrollment; 5. Individuals with recent intestinal surgery or severe intestinal diseases: patients suffering from severe intestinal conditions, such as severe intestinal infections (e.g. cholera, severe bacillary dysentery) or intestinal ischemic diseases; 6. Known or suspected allergy to lactulose or mannitol.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Serum metabolites;Fecal metabolites; | — |
Secondary
| Measure | Time frame |
|---|---|
| Area Under the Receiver Operating Characteristic Curve;Sensitivity;Specificity; | — |
Countries
China
Contacts
Public ContactWang Xinying
General Hospital of the Eastern Theater Command of the People's Liberation Army of China (Jinling Hospital Affiliated to Nanjing University Medical School)
Outcome results
None listed