Type 2 diabetic nephropathy
Conditions
Interventions
Non-DKD group:None
Sponsors
Zhejiang Provincial People's Hospital
Eligibility
Sex/Gender
All
Age
18 Years to 75 Years
Inclusion criteria
Inclusion criteria: 1. Patients aged 18–75 years; 2. Meet the diagnostic criteria for type 2 diabetes according to the 2022 "Guidelines for the Diagnosis and Treatment of Diabetes"; 3. Renal tissue pathology meets the diagnostic criteria for DKD; 4. Complete preservation of paraffin-embedded kidney tissue blocks.
Exclusion criteria
Exclusion criteria: 1. Patients with severe cardiovascular or cerebrovascular diseases; 2. Patients with other primary glomerular diseases; 3. Patients with autoimmune diseases such as systemic lupus erythematosus or Sjögren's syndrome; 4. Patients who have previously received glucocorticoid or immunosuppressive therapy; 5. Patients without complete clinical or pathological data.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Clinical data analysis (age, gender, duration of diabetes, HbA1c, eGFR, urine protein levels, serum creatinine, BUN, etc.);Histopathological analysis (using HE staining, PAS and Masson staining to observe the degree of glomerulosclerosis, mesangial matrix expansion, and tubular interstitial fibrosis);Protein localization and expression analysis (using immunohistochemistry (IHC) to detect the expression intensity and localization of proteins such as MECP2, SPI1, NLRP3, LC3, PINK1, and P62, with scoring based on staining intensity and positive area);Observation of mitochondrial morphology and structure (using transmission electron microscopy to observe the quantity, structure, cristae morphology, presence of vacuolization, and other pathological changes of mitochondria, and to assess autophagosome formation); | — |
Countries
China
Contacts
Public ContactQinghua Huang
Zhejiang Provincial People's Hospital
Outcome results
None listed