Study on Self-Driven Penetration into Dentinal Tubules and Long-Term Sealing Performance of Enzymatically Mineralized Chitosan Desensitizer

Status

Active, not recruiting

Phases

Early Phase 1

Study type

Interventional

Source

ChiCTR

Registry ID

ChiCTR2600116712

Enrollment

Unknown

Registered

2026-01-14

Start date

2026-01-25

Completion date

Unknown

Last updated

2026-01-27

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Dentin Hypersensitivity

Interventions

biofilm formation assessed using LIVE/DEAD staining and confocal microscopy.

Penetration depth experimental group:Treating EDTA-etched dentin slices with high, medium, and low concentrations of FITC-labeled chitosan nanocrystals (ChNCs), and evaluating fluorescence penetration

Sealing effectiveness experimental group:Dentin slices were divided into: control group, high-concentration repair group, low-concentration repair group, and mineralization solution group. Each group

the mineralization solution group received only CaGP

the control group received no treatment.

Air pressure penetration experimental group:Dentin slices were divided into: control group, high-concentration repair group, low-concentration repair group, GLUMA group, Aominqing group, and Duraphat

Bacterial adhesion and anti-biofilm experimental group:Experiment using Streptococcus mutans UA159. Part 1: Divided into control group, 12-h group, and 24-h group (5 slices per group)

bacterial adhesion observed by SEM. Part 2: Divided into control group, 24-h group, and 48-h group (5 slices per group)

Eligibility

Sex/Gender

All

Age

18 Years to 60 Years

Inclusion criteria

Inclusion criteria: 1. Patients aged 18 to 60 years..; 2. First, second, or third molars requiring extraction due to orthodontic treatment requirements, periodontal disease, or tooth impaction.

Exclusion criteria

Exclusion criteria: 1. Teeth with caries or cannot be fully extracted; 2. Teeth that have undergone root canal treatment or restoration.

Design outcomes

Primary

Measure	Time frame
Occlusion ratio;Occlusion depth;	—

Secondary

Measure	Time frame
Air pressure intensity;Bacterial density;	—

Countries

China

Contacts

Public ContactWang Guodong

Shanghai Changzheng Hospital

wangguodong@smmu.edu.cn+86 136 7198 9595

Outcome results

None listed

Source: ChiCTR (via WHO ICTRP) · Data processed: Feb 4, 2026