Bronchiectasis with acute exacerbation
Conditions
Interventions
Gold Standard:The final microbiological diagnosis was adjudicated based on a comprehensive clinical and microbiological evaluation, including BALF-tNGS results, conventional microbiological testing (C
Index test:Targeted Next-Generation Sequencing
Sponsors
Department of Respiratory and Critical Care Medicine, Key Laboratory of Respiratory Disease of Ningbo, The First Affiliated Hospital of Ningbo University, Ningbo
Eligibility
Sex/Gender
All
Age
18 Years to 80 Years
Inclusion criteria
Inclusion criteria: 1. Age >=18 years and <=80 years; 2. Chest computed tomography (CT) shows imaging features consistent with bronchiectasis; 3. Meet the diagnostic criteria for acute exacerbation of bronchiectasis, presenting with symptoms such as cough, sputum production, chest tightness, or wheezing, and willing to undergo bronchoscopy with bronchoalveolar lavage fluid (BALF) collection for tNGS testing; 4. Provide written informed consent and voluntarily participate in this clinical study.
Exclusion criteria
Exclusion criteria: 1. Presence of severe cardiopulmonary disease, coagulopathy, poor anesthesia tolerance, or psychiatric or severe neurofunctional disorders that constitute contraindications to bronchoscopy; 2. Intra-procedural desaturation (SpO2 decrease) or intolerance to bronchoscopy, or inability to tolerate further bronchoalveolar lavage despite tolerating bronchoscopy; 3. Inability to cooperate with study procedures for any reason, or deemed unsuitable for inclusion by the investigators for other medical or safety considerations; 4. Visible endobronchial hemorrhage on bronchoscopy suggesting potential risk of clinical deterioration following lavage; 5. Incomplete or missing key clinical information required for study assessment.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Diagnostic performance of BALF-tNGS compared with conventional microbiological tests; | — |
Secondary
| Measure | Time frame |
|---|---|
| Turnaround time of BALF-tNGS compared with conventional microbiological tests;Impact of BALF-tNGS and CMT on antimicrobial treatment decisions;Clinical improvement after treatment modification guided by BALF-tNGS and CMT;Pathogen spectrum identified by BALF-tNGS compared with conventional microbiological tests; | — |
Countries
Chian
Contacts
Public ContactChen Zhongbo
Department of Respiratory and Critical Care Medicine, Key Laboratory of Respiratory Disease of Ningbo, The First Affiliated Hospital of Ningbo University, Ningbo
Outcome results
None listed