Leucine perception/preference
Conditions
Interventions
Leucine-Deficient beverage intervention group:During the intervention period, subjects will receive leucine-deficient milk powder beverage (650-1000ml, to be consumed within 1 hour in divided doses) a
Control group:During the intervention period, subjects will receive control beverage (650-1000ml, to be consumed within 1 hour in divided doses) at: Breakfast (8:00-10:00), Lunch (11:30-13:00), Dinner
Sponsors
Fudan University
Eligibility
Sex/Gender
All
Age
20 Years to 40 Years
Inclusion criteria
Inclusion criteria: 1. Body mass index (BMI) between 18.5 and 24 kg/m²; 2. Aged 20 to 40 years; 3. In good general health with the ability to understand and comply with study requirements; 4. Voluntary participation with signed informed consent.
Exclusion criteria
Exclusion criteria: 1. History of diagnosed mental disorders; 2. Use of antibiotics or hormonal medications within the past two weeks; use of sedatives, hypnotics, antidepressants, anxiolytics, or antipsychotics within the past three months; history of alcohol abuse or other psychoactive substance abuse within the past three months; 3. Abnormal sense of smell or taste, or acute infectious diseases (e.g., influenza) that may temporarily affect smell or taste within the past week; 4. History of severe liver, kidney, endocrine, cardiovascular, gastrointestinal, pulmonary, hematological, metabolic diseases, or cancer; 5. History of major digestive system surgeries such as gastrectomy, gastrointestinal anastomosis, or intestinal resection (excluding appendectomy); 6. Current infectious diseases, including hepatitis B "Da San Yang" (HBeAg-positive), active tuberculosis, or HIV/AIDS; 7. Pregnant or lactating women; 8. Allergy to any component of the intervention formula (milk powder); 9. Serum leucine level below 80 µM.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Blood biochemical indicators;Serum amino acid;General control nonderepressible 2;Food preference;Beverage palatability;Beverage discrimination; | — |
Secondary
| Measure | Time frame |
|---|---|
| Anthropometric indices;Wearable device monitoring of sleep, stress, and physical activity;Dietary information;Demographic information;Genetic;Metabolomics;Circadian rhythm;Sleep quality;Gut microbiota;Proteomics;Resting metabolic rate;Mental status;Lifestyle;Medical history;Inflammatory markers;Transcriptomics;Methylation array;Environmental exposure markers;Oral microbiome;Gut barrier indicators;Nasal microbiome; | — |
Countries
China
Contacts
Public ContactLiang Sun
Fudan University
Outcome results
None listed