Sleep deprivation
Conditions
Interventions
Control Group:None
Acute sleep deprivation group:None
Sponsors
Southern Medical University Hospital of Integrated Traditional Chinese and Western Medicine
Eligibility
Sex/Gender
All
Age
20 Years to 40 Years
Inclusion criteria
Inclusion criteria: 1.Control Group: Age range: Healthy adults aged 20–40 years, with stable metabolic and immune function. Lifestyle: Maintained regular sleep patterns over the past three months (7–9 hours of sleep per night, bedtime <=11:00 PM). No history of chronic sleep deprivation (<=1 night of late sleeping per week), heavy alcohol consumption (alcohol intake <20 g/day for males, <10 g/day for females), or smoking. Diet control: Followed a standardized diet from two weeks before the study until its completion (to avoid interference with gut microbiota by high-fat or high-sugar diets). No gastrointestinal disorders: No history of inflammatory bowel disease, irritable bowel syndrome, chronic diarrhea, or constipation; no use of antibiotics, probiotics, or laxatives in the past month. Adequate sleep quality: Pittsburgh Sleep Quality Index (PSQI) score <=5; individuals with insomnia, sleep apnea, or other sleep disorders at baseline were excluded. 2.Acute sleep deprivation group: In addition to meeting all the above criteria, subjects have experienced recent acute sleep deprivation—defined as less than 5 hours of sleep per day over the past three days or continuous wakefulness for 24 hours.
Exclusion criteria
Exclusion criteria: 1. Medical History and Medication Use Liver diseases: Fatty liver, viral hepatitis (HBV/HCV positive), cirrhosis, or hepatic fibrosis. Metabolic diseases: Diabetes, obesity (BMI >=28), thyroid dysfunction, or autoimmune diseases. Mental health conditions: Depression, anxiety, or a history of using psychotropic medications (e.g., SSRIs). Recent infections: Acute infections within one month prior to the study (e.g., upper respiratory tract infections, gastroenteritis). 2. Exclusion of Confounding Factors Special diets: Vegetarians, individuals on ketogenic diets, or those recently participating in other clinical trials. Medication interference: Use of immunosuppressants, corticosteroids, or hepatotoxic drugs (e.g., acetaminophen) within three months prior to the study. Pregnancy/lactation: Not pregnant or lactating. 3. Additional Exclusion Criteria Non-compliance: Unable to complete the sleep deprivation intervention. Risk of sample contamination: Planning long-distance travel during the study period (interference due to jet lag) or requiring emergency medication. Genetic background interference: Family history of liver disease or hereditary abnormalities in gut microbiota (e.g., familial adenomatous polyposis).
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| ALT/ GPT;AST / GOT;TB;ALB;CRP;IL-6;TNF-a;TC;TG;FBG;16S rDNA gene ;PLT; | — |
Secondary
| Measure | Time frame |
|---|---|
| Hb;WBC; | — |
Countries
China
Contacts
Public ContactHe Songqi
Southern Medical University Hospital of Integrated Traditional Chinese and Western Medicine
Outcome results
None listed