Infection
Conditions
Interventions
Index test:mNGS sequencing results, positive culture rate, and positive pathogen identification
Sponsors
Shanghai Jiao Tong University Affiliated Sixth People's Hospital
Eligibility
Sex/Gender
All
Age
25 Years to 80 Years
Inclusion criteria
Inclusion criteria: 1. Treated in the Department of Neurosurgery of Shanghai Sixth People's Hospital, due to the condition of the need to be admitted to the intensive care unit, the age range is 18~80 years old; 2. Diagnosis of infection according to clinical symptoms, imaging results and routine blood tests; 3. Ability to obtain discarded cerebrospinal fluid (CSF), sputum, plasma and urine (UR) samples; 4. The sample was also sent for at least one routine test due to the need of the condition, including traditional bacterial culture; 5. Obtain the informed consent of the patient.
Exclusion criteria
Exclusion criteria: 1. Diagnosis and exclusion of infection; 2. Unable to obtain clinical samples; 3. The patient's baseline data is incomplete, and there are abnormal values in the baseline data; 4. The sample quality is unqualified (the library is not successfully established), and the mNGS quality control is not passed; 5. The patient refuses to give informed consent.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| mNGS squencing;culture results; | — |
Secondary
| Measure | Time frame |
|---|---|
| Complete blood count; | — |
Countries
China
Contacts
Public ContactChen Shiwen
Shanghai Jiao Tong University Affiliated Sixth People's Hospital
Outcome results
None listed