Suspicious ABO Blood Group Identification
Conditions
Interventions
Gold Standard:First generation sequencing technology
Index test:PACBIO third generation sequencing technology
Sponsors
The First Medical Center of Chinese PLA General Hospital
Eligibility
Sex/Gender
All
Age
1 Years to 100 Years
Inclusion criteria
Inclusion criteria: (1) Patients with inconsistent ABO blood group forward and reverse typing, antigenic attenuation (positive typing agglutination intensity =3+), mixed visual fields of positive typing , and antibody attenuation (reverse typing agglutination intensity <2+, and not able to reach 3+ after enhancement) on serological testing; (2) Patients and/or family members understand the treatment associated with this study and sign an informed consent form.
Exclusion criteria
Exclusion criteria: (1) Patients after ABO allogeneic haematopoietic stem cell transplantation; (2) Patients with no serological results that are not traceable; (3) Others who, in the judgement of the investigator, may confound the results of the study.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| First generation gene sequencing base mutations;PACBIO third-generation gene sequencing base mutations;Prediction of Alleles by First Generation Gene Sequencing;Prediction of Alleles by PACBIO Third Generation Gene Sequencing;Prediction of Phenotype by First Generation Gene Sequencing;Prediction of Phenotype by PACBIO third-generation gene sequencing;Consistency;Accuracy; | — |
Countries
China
Contacts
Public ContactYu Yang
The First Medical Center of Chinese PLA General Hospital
Outcome results
None listed