Deep-sited fungi infections
Conditions
Interventions
Gold Standard:This study enrolled patients with suspected deep-sited fungal infections and collected samples from the infected site. Deep fungal infections mainly include invasive fungal diseases (IFD
Index test:accuracy of fungal multiplex PCR-CRISPR system and Taqman probe microfluidic chip multiplex PCR :
Sensitivity, specificity, predictive value, likelihood ratio, and area under the ROC curve
Sponsors
Zhongshan hospital, Fudan University
Eligibility
Sex/Gender
All
Age
18 Years to 98 Years
Inclusion criteria
Inclusion criteria: 1.Age>18 years old 2. Experimental group included: patients diagnosed with confirmed/suspected IFD, CPA, Putative IFD, and available lesion specimens 3.Control group included: patients with hematological tumors receiving chemotherapy, granulocytopenia, immunosuppression (including solid organ transplantation or hematopoietic stem cell transplantation), or those with underlying lung diseases (including bronchiectasis, chronic obstructive pulmonary disease, etc.), as well as those with pulmonary infections or fever
Exclusion criteria
Exclusion criteria: 1.<18 years old; 2. Fungi cultures not submitted for testing 3. The specimen cannot meet the requirements for multiple PCR/CRISPR detection of fungi
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Sensitivity of CRISPR/cas12-PCR;Specificity of CRISPR/cas12-PCR; | — |
Secondary
| Measure | Time frame |
|---|---|
| Test the consistency of CRISPR/cas12-PCR and Taqman probe microfluidic chip multiplex PCR; | — |
Countries
China
Contacts
Public ContactHu bijie
Zhongshan hospital, Fudan University
Outcome results
None listed