Bloodstream infection
Conditions
Interventions
blood culture and Sanger sequencing
Index test:Detection results of the four target bacteria (E. coli, K. pneumoniae, A. baumannii, P. aeruginosa) by digital PCR technology
Sponsors
Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Be able to understand the research procedures, voluntarily participate and sign informed consent; 2. Clinicians suspect bloodstream infection; blood culture testing is required and any one of the following is met: 1) Meet the diagnostic criteria for sepsis: SOFA score increment = 2 points or qSOFA = 2 points (early stage); 2) Clinicians highly suspect bloodstream infection, and any one or more of the following clinical characteristics are present: a. Chills; b. Fever (body temperature >38.3?) or hypothermia (body temperature 12*10^9/L or WBC10%; d. PCT>2 times the upper limit of normal.
Exclusion criteria
Exclusion criteria: Anyone who meets any of the following conditions will be excluded: 1) Patients who have been enrolled in this clinical trial before; 2) Special groups such as patients with mental disorders and pregnant women; 3) Involuntarily participate in this study; 4) Samples that the researcher believes should be excluded.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Sensitivity;Specificity;Positive coincidence rate;Negative coincidence rate; | — |
Secondary
| Measure | Time frame |
|---|---|
| Total coincidence rate;Kappa index; | — |
Countries
China
Contacts
Public ContactYunsong Yu
Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University
Outcome results
None listed