Non-tuberculous mycobacterium lung disease
Conditions
Interventions
Gold Standard: Microbroth dilution
whole genome sequencing
Index test:Nanopore high-throughput targeted sequencing
Sponsors
Wenzhou Central Hospital
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Inclusion criteria
Inclusion criteria: 1. Age =18 years old, gender unlimited. 2. Meet the diagnostic criteria for suspected NTM lung disease, refer to China's "Guidelines for the Diagnosis and Treatment of Nontuberculous Mycobacteriosis 2020 Edition" : ? The sputum or bronchoalveolar lavage fluid is positive for acid-fast bacilli, but the clinical manifestations are inconsistent with tuberculosis; ? Abnormal mycobacteria were found in sputum or lavage fluid samples by microscope examination; ? The sputum or lavage fluid samples were positive for mycobacterium culture, but the colony morphology and growth were different from MTB. ? Sputum or lavage fluid samples were positive for acid-fast bacilli but negative for MTB molecular biology; ? Patients who received regular anti-tuberculosis therapy failed and repeated bacterial expulsion, and the lung lesions were mainly bronchiectasis, multiple nodules and parenchyma cavities; Cough cough sputum for more than 2 weeks, hemoptysis, wasting, fatigue, or night sweating have clinical symptoms of tuberculosis, but MTB molecular biological test and tuberculin skin test (TST) or Interferon-Gamma Release Assays (IGRAs) or recombinant tuberculosis fusion protein (ESAT6-CFP10, EC) are negative, imaging suspected NTM lung disease; ? Patients with pulmonary disease who have immune deficiency except tuberculosis. 3. The bronchoscopy physician assesses the patient's condition to tolerate alveolar lavage and obtain sufficient alveolar lavage fluid. 4. Subjects voluntarily participate in this study and sign informed consent; If the subject is unable to participate in the informed consent process, his/her legal representative must participate in the informed consent process and sign the informed consent.
Exclusion criteria
Exclusion criteria: 1. Not enough qualified bronchoalveolar lavage fluid specimens were obtained as expected. 2. Patients undergoing anti-mycobacterium therapy.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Species identification;Antimicrobial susceptibility;Accuracy;Sensitivity;Specificity; | — |
Countries
China
Contacts
Public ContactHe Guiqing
Wenzhou Central Hospital
Outcome results
None listed