Bullous skin disease
Conditions
Interventions
Gold Standard:ELISA results for BP180, BP230, Dsg1, and Dsg3
Index test:Nanofluorescence immunochromatography
Sponsors
Shanghai Skin Disease Hospital
Eligibility
Sex/Gender
All
Age
19 Years to 99 Years
Inclusion criteria
Inclusion criteria: Selection criteria for positive samples of bullous pemphigoid: ? The main manifestation is tension blisters on the basis of erythema on the skin, and Nielsen's sign is negative; ? The histopathology is a subepidermal blister with infiltration of inflammatory cells mainly composed of eosinophils in the dermis; ? Deposition of IgG and/or C3 in the patient's skin DIF basement membrane zone; ? Salt splitting indirect immunofluorescence examination showed linear deposition of immune complexes (IgG and/or C3) on the epidermal side; ? BP180 antibody test positive (ELISA value>9U/ml); Which conforms to ? and ?; Or if it meets ?, ?, ?+[? or ?], it can be selected Selection criteria for positive samples of pemphigus: ? Loose blisters appear on the skin and/or mucous membranes, with positive Nissl's sign; ? Histopathological examination revealed lysis cells in the spinous layer; ? Direct immunofluorescence showed deposition of IgG and/or C3 between keratinocytes; ? Dsg1 and/or Dsg3 ELISA values>20 U/ml. Conforming to ?+?+? or ?; ?+? Or ? or ? can be selected. Negative sample selection criteria: serum samples from other skin disease patients or healthy individuals without symptoms of bullous skin disease. All selected patients and healthy volunteers are required to sign an informed consent form.
Exclusion criteria
Exclusion criteria: None
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Sensitivity;specificity;accuracy; | — |
Secondary
| Measure | Time frame |
|---|---|
| ROC curve; | — |
Countries
China
Contacts
Public ContactPengfei Zhang
Shanghai Skin Disease Hospital
Outcome results
None listed