Diagnostic Value of Neutrophil Extracellular Net Trap in Sepsis

Status

Active, not recruiting

Phases

Early Phase 1

Study type

Observational

Source

ChiCTR

Registry ID

ChiCTR2300075650

Enrollment

Unknown

Registered

2023-09-11

Start date

2023-09-15

Completion date

Unknown

Last updated

2023-09-18

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Sepsis

Interventions

Gold Standard:Sepsis criteria (Sepsis3.0) : ?disorder of the body's response to infection and cause life-threatening organ dysfunction

? For ICU patients with suspected infection or infection, SOFA score increase of 2 points can be diagnosed

? For non-ICU patients with suspected infection or infection, qSOFA=2 points (systolic blood pressure =100 MMHG, respiratory rate =22 times/min, change of consciousness, 1 point for each one) can be d

Index test:? To detect the expression of plasma cell-free DNA (cfDNA) in neutrophil extracellular net traps in patients with sepsis, and its sensitivity and specificity

?To detect the expression of myeloperoxidase (MPO) in the neutrophil extracellular reticulum trap in septic patients, and its sensitivity and specificity

?detection in patients with sepsis neutrophils outer net trap to take off the amino acid acidification of histone H3 (Cit - H3) expression level, and its sensitivity and specificity. ? The differences

Eligibility

Sex/Gender

All

Age

19 Years to 79 Years

Inclusion criteria

Inclusion criteria: Sepsis group inclusion criteria: (1) Patients admitted to the Department of Emergency and Intensive Care Unit of Sun Yi Memorial Hospital of Sun Yat-sen University from August 1, 2023 to July 31, 2024; (2) Age: 19-79 years old; (3) voluntarily participate in and sign the informed consent. (4) Sepsis diagnostic criteria (Sepsis3.0) : for patients with infection or suspected infection, the diagnosis can be made when the patient's SOFA score increases by 2 points. Inclusion criteria for non-septic infection group: (1) Patients admitted to the Department of Emergency and Intensive Care Unit of Sun Yi Memorial Hospital of Sun Yat-sen University from August 1, 2023 to July 31, 2024; (2) Age: 19-79 years old; (3) voluntarily participate in and sign the informed consent. (4) Postadmission clinical data to determine the presence of infection, including culture results, molecular testing (e.g., polymerase chain reaction, antigen), relevant imaging, and histopathological examination. (5) Did not meet the diagnostic criteria for sepsis (Sepsis3.0) : the patient's SOFA score increased by less than 2 points.

Exclusion criteria

Exclusion criteria: 1: Cases without complete clinical data from August 1, 2023 to July 31, 2024 2: Pregnant or lactating women 3: The length of stay was less than 24 hours 4: Cancer patients with end-stage malignancy or undergoing chemotherapy or radiotherapy at presentation 5: HIV+ with CD4 count < 5×10^7/L, rheumatic disease or advanced disease (advanced hematological disease, advanced respiratory disease, advanced liver and kidney failure) 6: Missing data 7: Informed consent or authorization could not be obtained

Design outcomes

Primary

Measure	Time frame
Neutrophil extracellular net trap (NETs) markers: detection of free DNA;Neutrophils outer net trap (NETs) markers: myeloperoxidase (MPO) ;Marker of neutrophil extracellular net traps (NETs) : deacidulated amino acidified histone H3 (Cit-H3);Specificity;	—

Secondary

Measure	Time frame
Youden index in patients with sepsis diagnosed by NETs.;Positive predictive value of NETs in the diagnosis of sepsis.;Negative predictive value of NETs in the diagnosis of sepsis;Sensitivity;	—

Countries

China

Contacts

Public ContactFengqing Song

Sun Yat-sen Memorial Hospital, Sun Yat-sen University

songfq@mail.sysu.edu.cn+86 183 4432 7768

Outcome results

None listed

Source: ChiCTR (via WHO ICTRP) · Data processed: Feb 4, 2026