Intervertebral disc degeneration
Conditions
Interventions
Blank control group (part ?):None
PI3K/Akt agonist group (part ?):Agonizing the PI3K/AKT pathway with 740 Y-P
Normal nucleus pulposus cells group (part I):None
Abnormal nucleus pulposus cells group (part I):None
Blank control group (part II):None
PI3K/Akt inhibition group (part II):Inhibition of the PI3K/AKT pathway with LY294002
PI3K/Akt agonist group (part II):Agonizing the PI3K/AKT pathway with 740 Y-P
Empty plasmid transfection group (Part III):Transfection of nucleus pulposus cells with empty plasmids
Sox9 overexpression transfection group (Part III):Transfection of nucleus pulposus cells with SOX9 overexpression plasmid
Sox9 knockdown transfection set (Part III):Interference with SOX9 expression with siRNAs
PI3K/Akt agonist group+ Sox9 knockdown transfection set (part ?):Agonizing the PI3K/AKT pathway with 740 Y-P and Interference with SOX9 expression with siRNAs
COL2A1 overexpression group (part IV):Transfection of nucleus pulposus cells with COL2A1 overexpression plasmid
COL2A1 overexpression group+Sox9 knockdown transfection set (part IV):Transfection of nucleus pulposus cells with COL2A1 overexpression plasmid and Interference with SOX9 expression with siRNAs
PI3K/Akt agonist group+ COL2A1 knockdown transfection set (part ?):Agonizing the PI3K/AKT pathway with 740 Y-P and Interference with COL2A1 expression with siRNAs
Sponsors
Orthopedics Department of Affiliated Hospital of Zunyi Medical University
Eligibility
Sex/Gender
All
Age
18 Years to 60 Years
Inclusion criteria
Inclusion criteria: Inclusion criteria of abnormal nucleus pulposus cells : ? A total of 20 patients with LDH aged 18-60 years needed surgical treatment. ? All patients were routinely examined by X-ray, CT and MRI before operation. ? intervertebral discs were graded into Grade VI and V by Pfirrmann grading method. Inclusion criteria of normal nucleus pulposus cells : ? Patients who need surgical treatment for spinal trauma, slip or scoliosis; ? Patients who voluntarily choose intervertebral implant fusion surgical procedure and have surgical indications; ? Age =45 years; ?The intervertebral discs were graded as Grade ? and ? by Pfirrmann grading method.
Exclusion criteria
Exclusion criteria: Exclusion criteria for abnormal nucleus pulposus cells : No lower extremity neurological symptoms ( numbness, hypoesthesia or disappearance, decreased muscle strength, movement disorders, etc. ), no need for discectomy, spinal canal decompression cases. Exclusion criteria for normal nucleus pulposus cells : ?Those with abnormal blood routine, liver and kidney function, and coagulation function; ? pathological fractures due to tumor, tuberculosis, etc.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| SOX9 and COL2A1 mRNA expression in cells;Protein expression of SOX9 and COL2A1 in cells; | — |
Secondary
| Measure | Time frame |
|---|---|
| PI3K/AKT mRNA expression in cells;Protein expression of PI3K/AKT in cells; | — |
Countries
China
Contacts
Public ContactZhi-Jun Xin
Orthopedics Department of Affiliated Hospital of Zunyi Medical University
Outcome results
None listed