Respiratory infection
Conditions
Interventions
Gold Standard:The mixed results were determined from microbiologic tests (BALF culture, mNGS, nucleic acid-based testing, antigen and antibody detection, and pathological examination) performed within
Sponsors
Peking University People's Hospital
Eligibility
Sex/Gender
All
Age
18 Years to 100 Years
Inclusion criteria
Inclusion criteria: Patients with suspected pulmonary infection were subjected to bendable bronchoscopy. Metagenomic sequencing clinical tests were ordered during clinical diagnosis and treatment, and the remaining alveolar lavage fluid samples were detected to meet the 600µl required for tNGS detection. Complete basic clinical information was needed: subject name is replaced by digital code, gender, age, outpatient/hospitalization date, clinical diagnosis, blood routine results, clinical routine microbial culture results, clinical routine molecular detection results, clinical routine pathogen serum detection results
Exclusion criteria
Exclusion criteria: a. Samples that are not collected, processed, and stored in accordance with the requirements; b. Exclude samples with too little (less than 600 µl) remaining after meeting clinical routine tests c. Samples found to be contaminated before the test operation; d. Samples with incomplete clinical information.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Bacterial and fungal culture results;TB Xpert results;EB virus and cytomegalovirus nucleic acid test results;The clinician's assessment of the detected pathogen;GM results for plasma and BALF;Limit of Detection,LoD;accuracy;sensitivity;specificity; | — |
Countries
China
Contacts
Public ContactHui Wang
Peking University People's Hospital
Outcome results
None listed