Tuberculosis and nontuberculous mycobacteria
Conditions
Interventions
Patients infected with Mycobacterium tuberculosis group:No
Patients infected with non-tuberculous Mycobacteria group:No
Healthy Control group:No
Sponsors
Peking University People's Hospital
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: MTB patients, NTM patients, and healthy controls. Patients with MTB were expected to include 4-5 cases each of Mycobacterium intracellulace, Mycobacterium Kansas, Mycobacterium avium, Mycobacterium abscess, Mycobacterium Gordon, Mycobacterium toad and Mycobacterium tortoise. Inclusion Criteria: All patients were included as new-onset infection and did not have antituberculous disease prior to enrollment. MTB diagnosis adopts Shengxiang Biotechnology Co., Ltd. Mycobacterium tuberculosis nucleic acid detection kit (PCR-fluorescent probe method) (National Instrument Note 20173400166) combined with microbial culture. NTM was diagnosed by nucleic acid amplification, sequencing and microbial culture. (1) Two sputum samples sent separately for testing had positive NTM culture and were identified as homogeneous bacteria, and/or NTM molecular biology tests were all homogeneous bacteria; (2) Bronchial irrigation fluid or bronchoalveolar lavage fluid NTM culture and/or molecular biology test 1 time positive. Different NTMs were identified by amplification of 16S RNA-coding genes (16S DNA), 16S-23S rRNA intergenic regions (ITS), ß subunits (rpoB) of RNA polymerase and heat shock protein 65 (hsp65) coding genes.
Exclusion criteria
Exclusion criteria: Exclusion criteria for individuals with immunological abnormalities mainly include those with HIV infection, tumors, blood system diseases, rheumatic immune diseases, chronic kidney disease, rheumatic heart disease, Hashimoto's thyroiditis, allergic asthma, and diabetes. In addition, individuals with acute infectious diseases and pregnant women were also excluded.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| MTB and NTM testing of sputum or bronchial lavage fluid; | — |
Countries
China
Contacts
Public ContactZhancheng Gao
Peking University People's Hospital
Outcome results
None listed