changes of immune system
Conditions
Interventions
Time-restricted eating group :Intervention eating time: three well-balanced meals with slightly more than the daily energy requirement are provided at 9AM to 5PM on a 24-hour basis for 30 days.
Ramadan fasting group:NA
Sponsors
The Second Xiangya Hospital of Central South University
Eligibility
Sex/Gender
All
Age
18 Years to 60 Years
Inclusion criteria
Inclusion criteria: 1. 18-60 years; 2. Body mass index (BMI) is between 18.0 and 27.0 kg/m2; 3. Body weight was stable (change<± 10%) 3 months before the study, without metabolic disorder or cardiovascular disease history; 4. There is no acute or chronic inflammation. At present, no drugs are used to regulate blood sugar, blood pressure, blood lipids or hormones; 5. Do not drink too much alcohol (more than 15 cups per week); 6. No dietary taboos (e.g. vegetarians); 7. No gastrointestinal diseases.
Exclusion criteria
Exclusion criteria: 1. Have a tendency to eat disorders; 2. Malignant tumor confirmed by biopsy; 3. Women who are pregnant, nursing or preparing for pregnancy; 4. Any chronic disease history that may affect the interpretation of the research results; 5. Women or men take hormone supplements or anti pregnancy hormone drugs for more than 2 months; 6. Have received antidepressant drugs in the past 6 months; 7. Take non steroidal anti-inflammatory drugs (NSAIDs) regularly.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| CD4+CD27-CD28- senescent T cell frequencies in peripheral blood mononuclear cells (PBMCs); | — |
Secondary
| Measure | Time frame |
|---|---|
| weight changes and onther weight related index: body moisture, skeletal muscle, body fat rate, BMI, muscle mass, visceral fat, bone mass, basal metabolism and fat weight. ;Frequencies of each immunocytes and immunosenescent cells in PBMC. Flow cytometry detection of changes in the frequencies of following immunocytes: (a) each subset of T cells (CD4+ T cells, CD4+ Naive T cells, CD4+ Effector T cells, Th1, Th2, Th17, Treg, Tfh-like, CD8+ T cells, CD8+ Naive T cells, CD8+ Memory T cells). (b) B cell subpopulation (total CD19+ B cells, Naive B cells, Memory B cells, and plasma cells). (c) CD27-CD28- immunosenescent cells in each subset.;Serum metabolomics: LC–MS metabolomic analysis was applied to detect changes in serum metabolites.; Immune repertoire:immune repertoire refers to the sum of all functionally versatility T cells and B cells in the circulattion system of individuals.; Gut microorganism: 16S rDNA sequencing analysis was applied to detect changes in intestinal microecology.; | — |
Countries
China
Contacts
Public ContactHai Long
Department of Dermatology, The Second Xiangya Hospital of Central South University
Outcome results
None listed