Leukemia
Conditions
Interventions
None listed
Sponsors
Affiliated Hospital of Zunyi Medical University
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: Children who can clearly diagnose leukemia can be included 1. The basic diagnostic basis for AML diagnosis: 1). Clinical symptoms and signs: fever, pallor, fatigue, bleeding, bone and joint pain, enlargement of liver, spleen, lymph nodes and other infiltrating lesions. 2). Hemogram changes: hemoglobin and red blood cells are decreased, platelets are decreased, white blood cells are increased, normal or decreased, and different numbers of prokaryotic, young granulocyte (or young monocyte) cells or no prokaryotic, young granulocyte (or young monocyte) cells can be found by classification. 3). Morphological changes of bone marrow: it is the main basis for diagnosis: most of the nucleated cells in the bone marrow smear show obvious hyperplasia or extreme hyperplasia, and only a few show hypoplasia, which is mainly myeloid cell hyperplasia. AML can be diagnosed only when the number of promyelocytes+promyelocytes (or promyelocytes+promyelocytes) cells is = 20%. 2. Red leukemia (M6): In addition to the above, there are erythroid = 50% with morphological abnormalities; The megakaryocytes in bone marrow of acute megakaryocytic leukemia (M7) = 30%. In addition to the Rexhlet staining classification counting of bone marrow smears and observation of cell morphological changes, cytochemical staining tests such as peroxidase (POX), glycogen (PAS), non-specific esterase (NSE) and sodium fluoride esterase (NaF) inhibition tests should be performed to further determine the nature of abnormal cells and differentiate them from acute lymphoblastic leukemia (ALL). 3. ALL diagnostic criteria: All suspected cases should be diagnosed and classified by morphology immunology cytogenetics molecular biology (MICM), and should meet one of the following criteria: 1) Bone marrow morphology criteria: according to WHO 2016 diagnostic criteria, the original and immature lymphocytes in bone marrow should be = 20%. 2) If the proportion of immature cells is less than 20%, molecular diagnosis must be made to confirm the presence of ALL pathogenic gene, such as ETV6-RUNX1. The diagnosis can be made when the proportion of primary and immature lymphocytes in bone marrow smear exceeds 20%. 4. CML diagnostic criteria: Typical clinical manifestations, combined with positive Ph chromosome and/or BCR-ABL fusion gene, can confirm the diagnosis.
Exclusion criteria
Exclusion criteria: 1.Bone marrow samples could not be collected during the patient's diagnosis and treatment.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| ZNF622 gene and protein levels; | — |
Countries
China
Contacts
Public ContactChen Yan
Affiliated Hospital of Zunyi Medical University
Outcome results
None listed