N/A
Conditions
Interventions
Trauma group :Follow trauma guidelines
Control group:None
Sponsors
Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
Eligibility
Sex/Gender
Male
Age
18 Years to 50 Years
Inclusion criteria
Inclusion criteria: 1. Trauma group: (1) Gender: male; (2) Aged 18-50 years old; (3) Patients with severe closed fracture of lower extremity; (4) ISS>=16 points. 2. Control group (healthy volunteers): (1) Gender: male; (2) Aged 18-50 years old; (3) Good health, no history of heart, liver, kidney, digestive tract, nervous system, mental disorders and metabolic disorders; (4) No immune-related diseases, and no immune-enhancing or immunosuppressive drugs have been used before; (5) Sign the informed consent.
Exclusion criteria
Exclusion criteria: 1. Trauma group (with one or more of the following conditions): (1) Patients with open injury; (2) Patients on long-term hormone or immunosuppressive therapy; (3) Those with a history of immunodeficiency, including HIV positive or rheumatoid arthritis, lupus erythematosus, myasthenia gravis, chronic glomerulonephritis and other basic chronic diseases of the immune system, or a history of organ transplantation; (4) Fever (body temperature >38.5?) on admission, accompanied by increased white blood cell count and increased erythrocyte sedimentation rate; (5) The patient's survival time is less than 7 days. 2. Control group (with one or more of the following conditions): (1) Abnormal blood pressure, electrocardiogram, respiratory status or liver and kidney function, and routine blood and urine routine examinations (judged by clinicians to have clinical significance); (2) History of alcoholism, smoking, drug abuse or drug use in the past year; (3) Participated in drug trials or used related immune preparations within three months before enrollment; (4) Have used any other drugs within two weeks before the start of the trial; (5) Inability to tolerate venipuncture for blood collection; (6) Those who are participating in other clinical experiments.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Tim-3 positive peripheral blood NKT cells;IL-12;Apoptosis NKT cells;Interferon-r;IL-6;IL-10;Tim-3 positive peripheral blood NK cells;Apoptosis NK cells;Tim-3 positive peripheral blood CD4+T cells;ApoptosisCD4+T cells;Tim-3 positive peripheral blood CD8+T cells;ApoptosisCD8+T cells;High-sensitivity C-reactive protein;High-sensitivity troponin-I;Cystatin C;Lactic acid;Alanine aminotransferase;Aspartate aminotransferase;Prothrombin time;Activated partial thromboplastin time;D-D dimer;International normalized ratio;NT-proBNP;Procalcitonin;White blood cell count;Platelet count;Prealbumin;Albumin;IL-8;TNF-alpha;IL-2r;IL-1 beta;Injury severity score;Acute physiology and chronic health score-II;Brief damage grading criteria;Glasgow coma score;Age;Sex;Heart rate;Blood pressure;Body temperature;Arterial blood gas;IL-23;IL-27;IL-18;Tim-3 negative peripheral blood NKT cells;Apoptosis Tim-3 positive peripheral blood NKT cells;Apoptosis Tim-3 negative peripheral blood NKT cells; | — |
Countries
China
Contacts
Public ContactTang Zhaohui
Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
Outcome results
None listed