Gynecological oncology, endometriosis, breast cancer, hemopathy, etc.
Conditions
Interventions
Group 1:Fresh ovarian tissue is stored at low temperature for 24 hours in vitro
Group 2:Fresh ovarian tissue is stored at low temperature for 30 hours in vitro
Group 3:Fresh ovarian tissue is stored at low temperature for 48 hours in vitro
Sponsors
Beijing Obstetrics and Gynecology Hospital, Capital Medical University
Eligibility
Sex/Gender
Female
Inclusion criteria
Inclusion criteria: 1. In principle, the age is not more than 37 years old, the age limit can be appropriately relaxed according to the ovarian reserve function and personal wishes. 2. As a result of the primary disease and its treatment, short-term need to receive radiotherapy, chemotherapy or other patients who may cause a high risk of POI. 3. The prognosis of primary disease is good, and the 5-year survival rate is higher. 4. At least 3 days before the beginning of radiotherapy and chemotherapy. 5. Can tolerate laparoscopic or open ovarian tissue extraction 6. The patient signed the informed consent form.
Exclusion criteria
Exclusion criteria: 1. The function of ovarian reserve is low, and POI has occurred before cryopreservation. 2. Patients with malignant ovarian tumors or suspected primary ovarian metastasis by imaging. 3. The patient's physical condition is not good, and can not tolerate the surgery of ovarian tissue extraction.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| follicle viability;follicle morphology;cell apoptosis; | — |
Secondary
| Measure | Time frame |
|---|---|
| Tumor necrosis factor a;B-cell lymphoma-2;Bone Morphogenetic Protein 15;Growth Differentiation Factor 9;estradiol;progesterone;Anti-mullerian hormone;Glucose uptake rate; | — |
Countries
China
Contacts
Public ContactRuan Xiangyan
Beijing Obstetrics and Gynecology Hospital, Capital Medical University
Outcome results
None listed