Gastric cancer
Conditions
Interventions
Gold Standard:The expression of HER2 was confirmed by immunohistochemistry and FISH in pathological specimens of gastric cancer patients.
of 
gene 
in 
peripheral 
blood 
ctDNA
Sponsors
Shenzhen Nanshan People's Hospital
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: (1) Seventy-five patients with gastric cancer admitted to Nanshan Hospital underwent open or laparoscopic surgery and were diagnosed as gastric cancer by histopathology. According to the TNM staging standard (2016, eighth edition) published by the International Anti-Cancer Alliance/United States Joint Cancer Committee (UICC/AJCC), the primary lesions were determined by size, depth of invasion, and lymph nodes. Stage I-IV staging was performed under the condition of degree of obstinate metastasis and distant metastasis. All patients did not receive any chemotherapy, radiotherapy or immunotherapy before operation. After informed consent, 37 healthy volunteers were selected as controls in the same period; (2) Thirty patients with TNM stage III and IV advanced gastric cancer (at least 30 cases) were selected to be included in the dynamic monitoring study. The abnormal amplification of HR2 gene in peripheral blood was detected before operation, one month after operation and every three months, and the clinical characteristics such as imaging and therapeutic reactivity were recorded in detail; (3) IHC and FISH were performed in all patients with gastric cancer. According to the "Guidelines for HER2 Detection of Gastric Cancer (2016 edition)", IHC was detected in the tissue samples first. IHC score of 3 + was judged to be HER2 positive, IHC score of 0 and 1 + was judged to be negative, and IHC score of 2 + was judged to be HER2 positive. The situation.
Exclusion criteria
Exclusion criteria: No
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Detection of HER2 gene FISH in pathological specimens;Immunohistochemical detection of HER2 gene in pathological specimens;Her2 ctDNA Abnormal amplification; | — |
Countries
China
Contacts
Public ContactJihui Du
Shenzhen Nanshan People's Hospital
Outcome results
None listed