Health Condition 1: B968- Other specified bacterial agents as the cause of diseases classified elsewhere
Conditions
Interventions
Intervention1: NIL: NIL
Intervention2: Nil: Nil
Control Intervention1: NIL: NIL
Sponsors
None
Eligibility
Inclusion criteria
Inclusion criteria: 1.A well-characterized, catheter-associated P. aeruginosa strain capable of biofilm formation and resistant to levofloxacin, cotrimoxazole, and minocycline. 2.A catheter-associated S. maltophilia strain capable of biofilm formation and susceptible to levofloxacin, cotrimoxazole, and minocycline. 3.ICU patients diagnosed with catheter-associated infections involving S. maltophilia with or without P. aeruginosa based on routine clinical microbiology reports from same site.
Exclusion criteria
Exclusion criteria: 1.Mixed cultures with more than 2 organisms. 2.Non-catheter-associated clinical source. 3.Poor viability or contamination failed isolates.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To evaluate the impact of P. aeruginosa on the antibiotic susceptibility (minimum biofilm eradication concentration) of Catheter-associated- S. maltophilia to levofloxacin, cotrimoxazole (SXT) and minocycline within dual-species (S. maltophilia & P. aeruginosa) biofilms.Timepoint: Each sample will be assessed at 3 different time-points (24, 48 and 72 hours) after inoculation | — |
Secondary
| Measure | Time frame |
|---|---|
| To quantify the biofilm biomass & architecture of mono- & dual- species biofilms of Stenotrophomonas maltophilia with or without Pseudomonas aeruginosa.Timepoint: Each sample will be assessed at 3 different time-points (24, 48 & 72 hours) after inoculation;To quantify antibiotic penetration within mono- & dual- species biofilms of Stenotrophomonas maltophilia with or without Pseudomonas aeruginosa.Timepoint: Each sample will be assessed at 3 different time-points (24, 48 & 72 hours) after inoculation;To identify genomic differences in Stenotrophomonas maltophilia from mono- versus dual-species biofilms with Pseudomonas aeruginosa, associated with discordant susceptibility to minocycline, levofloxacin, & cotrimoxazole.Timepoint: Each sample will be assessed at 3 different time-points (24, 48 & 72 hours) after inoculation;To evaluate the real-world effectiveness of antibiotic treatment in catheter-associated infections caused by Stenotrophomonas maltophilia mono-species & Stenotrophomonas maltophilia Pseudomonas aeruginosa dual-species biofilms by correlating microbiological eradication rates with in-vitro antibiotic penetration & susceptibility.Timepoint: From patient admission to 28 days post-admission or death or discharge whichever occurs earlier | — |
Countries
India
Contacts
Public ContactDr Gaurav Jain
All India Institute Of Medical Sciences(AIIMS), Rishikesh
Outcome results
None listed