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Sperm washing of samples and effect on DNA survival for Intra uterine insemination

Impact of Post Wash Incubation Time on Sperm DNA Integrity in Normal and Abnormal Samples - NIL

Status
Recruiting
Phases
Unknown
Study type
Interventional
Source
CTRI
Registry ID
CTRI/2026/03/106589
Enrollment
30
Registered
2026-03-19
Start date
Unknown
Completion date
Unknown
Last updated
2026-03-30

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Interventions

Control Intervention1: Abnormal sperm samples: Normal sperm samples and their comparing with oligozoospermic sperm samples (meaning sperm concentration less than 16 million/ml as per WHO guidelines 20

Sponsors

Amandeep Kaur
Lead Sponsor

Eligibility

Inclusion criteria

Inclusion criteria: normozoospermic and oligozoospermic semen samples which is Normozoospermic - having normal sperm concentration, motility as per WHO 2021 guidelines and oligozoospermic is less than the referecnes lower limit of sperm concentration of WHO 2021

Exclusion criteria

Exclusion criteria: Asthenozoospermia - sperm motility less than the referenced limit of WHO 2021 guidelines , azoospermia - no sperm seen in the ejaculate referenced by WHO 2021 guidelines and surgically retrieved sperm by testicular tissue

Design outcomes

Primary

MeasureTime frame
To identify and recover sperm with intact DNA after processing, determine the best time to use them for IUITimepoint: The sperm sample assessment which will be of each sample and the assessment will be in hours only. that is 0 hour, 4 hour and 24 hour. the study will be done for all samples for 8 weeks

Secondary

MeasureTime frame
NILTimepoint: NIL

Countries

India

Contacts

Public ContactAmandeep Kaur

Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka

sonia.kmcmpl2024@learner.manipal.edu9632223037

Outcome results

None listed

Source: CTRI (via WHO ICTRP) · Data processed: Apr 4, 2026