A Study to Compare Embryo Development from Sibling Eggs Using ICSI in Different Culture Media

Assessment of Embryo Developmental Outcomes from Sibling Oocytes Subjected to ICSI in Sodium Bicarbonate-Buffered Versus HEPES-Buffered Media - NIL

Status

Active, not recruiting

Phases

Phase 4

Study type

Interventional

Source

CTRI

Registry ID

CTRI/2026/02/104230

Enrollment

Registered

2026-02-18

Start date

Unknown

Completion date

Unknown

Last updated

2026-03-02

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Interventions

Intervention1: ICSI in Sodium Bicarbonate-Buffered Culture Medium: Sibling oocytes will undergo intracytoplasmic sperm injection (ICSI) followed by embryo culture in sodium bicarbonate-buffered medium

Eligibility

Inclusion criteria

Inclusion criteria: Women between the ages of 25-35 years

Exclusion criteria

Exclusion criteria: Severe male factors and poor ovarian response ( less than 5 oocytes)

Design outcomes

Primary

Measure	Time frame
To compare embryo developmental outcome i.e blastocyst formation rate between sibling oocytes subjected to ICSI (bicarbonate-buffered medium, and HEPES-buffered medium.)Timepoint: Baseline (Day 0): At the time of intracytoplasmic sperm injection (ICSI) and allocation of sibling oocytes to sodium bicarbonate-buffered or HEPES-buffered media. Day 1 (16 18 hours post-ICSI): Assessment of fertilization outcome. Day 3 (68 72 hours post-ICSI): Assessment of cleavage-stage embryo development and quality. Day 5/6 post-ICSI : Assessment of blastocyst formation and quality.	—

Measure

Time frame

To compare embryo developmental outcome i.e blastocyst formation rate between sibling oocytes subjected to ICSI (bicarbonate-buffered medium, and HEPES-buffered medium.)Timepoint: Baseline (Day 0): At the time of intracytoplasmic sperm injection (ICSI) and allocation of sibling oocytes to sodium bicarbonate-buffered or HEPES-buffered media. Day 1 (16 18 hours post-ICSI): Assessment of fertilization outcome. Day 3 (68 72 hours post-ICSI): Assessment of cleavage-stage embryo development and quality. Day 5/6 post-ICSI : Assessment of blastocyst formation and quality.

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Secondary

Measure	Time frame
To compare fertilization rates (2PN formation) between the two media. To assess cleavage, blastomere symmetry, fragmentation, & multinucleation were assessed. Blastocyst formation rate, expansion grade, ICM grade, & TE grade were evaluated. To determine whether transient exposure to HEPES during ICSI affects downstream embryo viability and usability rates. To generate evidence for refining standard operating procedures in ICSI laboratories.Timepoint: Baseline (Day 0): At the time of intracytoplasmic sperm injection (ICSI) & allocation of sibling oocytes to sodium bicarbonate-buffered or HEPES-buffered media. Day 1 (16 18 hours post-ICSI): Assessment of fertilization outcome. Day 3 (68 72 hours post-ICSI): Assessment of cleavage-stage embryo development & quality. Day 5/6 post-ICSI : Assessment of blastocyst formation & quality.	—

Measure

Time frame

To compare fertilization rates (2PN formation) between the two media. To assess cleavage, blastomere symmetry, fragmentation, & multinucleation were assessed. Blastocyst formation rate, expansion grade, ICM grade, & TE grade were evaluated. To determine whether transient exposure to HEPES during ICSI affects downstream embryo viability and usability rates. To generate evidence for refining standard operating procedures in ICSI laboratories.Timepoint: Baseline (Day 0): At the time of intracytoplasmic sperm injection (ICSI) & allocation of sibling oocytes to sodium bicarbonate-buffered or HEPES-buffered media. Day 1 (16 18 hours post-ICSI): Assessment of fertilization outcome. Day 3 (68 72 hours post-ICSI): Assessment of cleavage-stage embryo development & quality. Day 5/6 post-ICSI : Assessment of blastocyst formation & quality.

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Countries

India

Contacts

Public ContactSimran Kochhar

Akanksha IVF, Room 628, Mata Chanan Devi Hospital

simrankochhar123@gmail.com9646943744

Outcome results