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Testing Simple Home Remedies to Remove Germs From Used Toothbrushes

Evaluation of Microbial Contamination of Toothbrushes and their Decontamination Using Various Home Remedies : An Ex Vivo Study

Status
Recruiting
Phases
Unknown
Study type
Interventional
Source
CTRI
Registry ID
CTRI/2026/01/102007
Enrollment
30
Registered
2026-01-23
Start date
Unknown
Completion date
Unknown
Last updated
2026-03-02

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Interventions

Intervention1: Nil: Nil Intervention2: Vinegar Group: Toothbrush immersed in 50% white vinegar(10ml) solution for 10 minutes and then allowed to air dry Intervention3: Baking Soda + Lemon Group: Tooth

Sponsors

Dr Preksha Parakh
Lead Sponsor

Eligibility

Inclusion criteria

Inclusion criteria: Subjects between the age of 22 30 years with at least 20 natural teeth Normal and healthy patients with no systemic disease

Exclusion criteria

Exclusion criteria: Subjects on antibiotics and antimicrobials any ongoing dental treatment smoking systemic diseases and subjects lacking dexterity who cannot follow brushing techniques.

Design outcomes

Primary

MeasureTime frame
Reduction in microbial contamination of toothbrushes, expressed as total viable bacterial count (CFU/mL) recovered from used toothbrushes after disinfection with: 1.Tap water 2. Mouthrinse 3.White vinegar 4. White vinegar + mouthrinse 5.Baking soda + lemon solutionTimepoint: 1.Day 7- Sample Collection I(Tap water) 2.Day 14- Sample Collection II (Mouthrinse) 3.Day 15-22 -Washout period 4. Day 29 - Sample Collection III (White vinegar) 5. Day 30-37- Washout period 6.Day 44- Sample collection IV (White vinegar+cholrhexidine) 7. Day 45-52 - Wahout period 8.Day 58-Sample Collection V (Baking soda +lemon)

Secondary

MeasureTime frame
1.Comparative percentage reduction in microbial load between the disinfectants. 2.Participant compliance and feasibility of using natural disinfectants for routine toothbrush hygiene.Timepoint:

Countries

India

Contacts

Public ContactAnshula N Deshpande

Sumandeep Vidyapeeth Deemed to be University, Vadodara

dranshula@gmail.com7600916400

Outcome results

None listed

Source: CTRI (via WHO ICTRP) · Data processed: Mar 14, 2026