For root canal treatment, which irrigant removes more bacteria and causes less pain? Chlorhexidine or a peptide GH12

Comparative evaluation of microbial load reduction and post operative pain assessment of antimicrobial peptide GH12 and chlorhexidine as root canal irrigants-A randomised controlled trial - NIL

Status

Active, not recruiting

Phases

Phase 4

Study type

Interventional

Source

CTRI

Registry ID

CTRI/2025/12/100000

Enrollment

Registered

2025-12-31

Start date

Unknown

Completion date

Unknown

Last updated

2026-01-12

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Health Condition 1: K029- Dental caries, unspecified

Interventions

Intervention1: Peptide GH12: 5ml of Antimicrobial Peptide GH12, concentration of 3.2mg/100 ml, will be used as the final irrigant Control Intervention1: Chlorhexidine: 5ml of 2 percent chlorhexidine w

Eligibility

Inclusion criteria

Inclusion criteria: 1. Patients with a single-rooted tooth with a single canal and pulp necrosis, which will be confirmed by the absence of a response to pulp sensibility tests and radiographic evidence of apical periodontitis or periapical radiolucency. 2. No spontaneous pre-treatment pain at the time of the appointment.

Exclusion criteria

Exclusion criteria: 1. Patients who used antibiotics in the past three months or had systemic disease. 2. Pregnant women or lactating mothers. 3. History of any previous drug reaction. 4. Visibly exposed root canals. 5. Teeth that could not be isolated. 6. Fistula and edema in periapical tissues. 7. Advanced periodontal disease. 8. Incomplete root formation. 9. History of recent dental trauma. 10. Teeth with root canal curvatures greater than 20 . 11. Root shorter than 15mm and longer than 25mm. 12. Crown/root fracture, root resorption, or calcifications.

Design outcomes

Primary

Measure	Time frame
Microbial load reductionTimepoint: Sample 1-After access opening and before cleaning and shaping Sample 2-After irrigation with sodium hypochlorite and EDTA Sample 3-After final irrigation	—

Secondary

Measure	Time frame
Postoperative painTimepoint: 24 hours 48 hours 72 hours and 5th day	—

Countries

India

Contacts

Public ContactDr Sanghamitra Sagariya

SCB DENTAL COLLEGE AND HOSPITAL, CUTTACK, ODISHA

amitjena@yahoo.com9437836499

Outcome results

None listed

Source: CTRI (via WHO ICTRP) · Data processed: Feb 4, 2026