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Role of flow cytometry in differentiating between the body s first line of defense (innate immunity) and its learned defense (adaptive immunity) in children.

Role of flow cytometry in distinguishing between innate immunity and adaptive immunity using (m)CD169 and (n)CD64 in paediatric population. - NIL

Status
Active, not recruiting
Phases
Unknown
Study type
Observational
Source
CTRI
Registry ID
CTRI/2025/12/098248
Enrollment
20
Registered
2025-12-01
Start date
Unknown
Completion date
Unknown
Last updated
2025-12-08

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Health Condition 1: B95-B97- Bacterial and viral infectious agents

Interventions

Intervention1: Nil: Nil Intervention2: Nil: Nil Intervention3: Nil: Nil Intervention4: Nil: Nil

Sponsors

Maulana Azad Medical College
Lead Sponsor

Eligibility

Inclusion criteria

Inclusion criteria: All paediatric patients in the department of Paediatrics of 01 month to 05 years of age who present to OPD or emergency department within 05 days of acute infective illness, with any or all symptoms like cold, cough, coryza, wheeze, fever, myalgia, arthralgia, rashes, diarrhoea, vomiting and headache, with having no prior antibiotic intake when presenting to the OPD or the Emergency wards.

Exclusion criteria

Exclusion criteria: 1. Patients presenting with any etiology other than infections. 2. Patients presenting with chronic infections, Tubercular, Malarial and Fungal infections. 3. Patients who have received antibiotics.

Design outcomes

Primary

MeasureTime frame
Proportion of cells expressing mCD169 and nCD64 in monocytes and neutrophils by flow cytometry in paediatric population in infections.Timepoint: Baseline

Secondary

MeasureTime frame
Correlation of mCD169 with innate immune response for viral infections and nCD64 with adaptive immune response for bacterial infections using various parameters: 1)ELISA, PCR, Cultures. 2) C Reactive protein, Procalcitonin, LDH. 3) Total leukocyte count, Absolute Neutrophil and Absolute Lymphocyte count.Timepoint: Baseline

Countries

India

Contacts

Public ContactDr Sarika Singh

Maulana Azad Medical College

sarikasingh97b@gmail.com9911182326

Outcome results

None listed

Source: CTRI (via WHO ICTRP) · Data processed: Feb 4, 2026