Health Condition 1: K029- Dental caries, unspecified
Conditions
Interventions
Intervention1: group 1 ECC with swelling/abscess (extraoral or intraoral)
Group 2 ECC with multiple decayed teeth more than 10
Group 3 ECC with multiple decayed teeth less than 10
: Clinical & History
Sponsors
saveetha dental college
Eligibility
Inclusion criteria
Inclusion criteria: Diagnosed with Early Childhood Caries (ECC) based on AAPD criteria. Study groups: Group 1: ECC with clinical signs of swelling (e.g., dental abscess or localized soft tissue inflammation). Group 2: ECC without swelling. Group 3: Caries-free, healthy children (control group). Medically healthy with no systemic or immune-compromising diseases. Informed written consent obtained from parents or legal guardians.
Exclusion criteria
Exclusion criteria: Presence of systemic illness (e.g., diabetes, cardiac disorder or immunodeficiency diseases). History of congenital anomalies or genetic disorders. Teeth indicated due to trauma or developmental defects unrelated to caries. Parents/guardians refusing consent or unwilling to comply with study procedures.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Evaluation of the expression levels of Proliferating Cell Nuclear Antigen PCNA in children with early childhood caries compared to caries free children Assessment of anti inflammatory cytokine markers such as Interleukin 10 IL10 and Transforming Growth Factor Beta TGF beta in both study and control groups Detection and quantification of gene expression through Polymerase Chain Reaction PCR analysis Immunohistochemical analysis of tissue samples to localize and quantify PCNA and anti inflammatory marker expression Correlation of molecular and immunohistochemical findings with the clinical severity of early childhood cariesTimepoint: 6 months | — |
Secondary
| Measure | Time frame |
|---|---|
| Correlation between PCNA and cytokine expression levels to explore the relationship between cell proliferation and immune regulation in ECC. Assessment of the association between biomarker expression levels and clinical caries severity (e.g., dmft/dmfs scores) for potential diagnostic or prognostic value. Timepoint: 6 months | — |
Countries
India
Contacts
Public ContactRamesh R
Saveetha dental college
Outcome results
None listed