None listed
Conditions
Interventions
Intervention1: Nil: Nil
Intervention2: Application of multimodal Raman tweezers: Participants red blood cells will be analyzed using multimodal Raman tweezers, a non-invasive technique that combines o
Intervention3: Nil: Nil
Control Intervention1: Natural aging Process: Since the study involves drawing 4ml of intravenous blood, and only a part of the leftover blood sample will be used for the study
Sponsors
Manipal Academy of Higher Education (MAHE), Manipal
Eligibility
Inclusion criteria
Inclusion criteria: Age Range: To facilitate a comparative analysis of aging biomarkers, participants will be selected from distinct Age groups, including young adults (20-39 years), middle-aged individuals (40-59 years), and the elderly (60+ years). We will consider 30 men and 30 women in each group of 60 participants above. Hemoglobin level: Participants with a minimum hemoglobin level requirement. Male: 13.2 to 16.6 grams per deciliter (g/dL) Female: 11 to 14 g/dL (on average).
Exclusion criteria
Exclusion criteria: Participants undergoing recent medical treatments (e.g., chemotherapy, immunosuppressive therapy, blood transfusions, or vaccinations), those with blood disorders (like anemia or HIV), diabetic patients, and individuals with genetic syndromes affecting blood will be excluded
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| These are the prominent measures used to study the usefulness, associations between the prominent variables, and groups or themes generated at the end of the study. 1. The study will generate a comprehensive Raman spectral dataset from RBCs of individuals spanning different ages. The research is expected to elucidate specific markers indicative of cellular aging by correlating these spectral variations with age. 2. The analysis will provide insights into how Raman spectral variations relate to biochemical parameters for aging. The study will deepen our understanding of how human aging impacts the functionality and detection of molecular and structural alterations in RBCs. 3. Using Raman tweezer setups is a new way to study how individual cells behave as they age. The success of this technology could lead to more advanced experimental designs in future blood-related research. Timepoint: 3 years | — |
Secondary
| Measure | Time frame |
|---|---|
| NILTimepoint: NIL | — |
Countries
India
Contacts
Public ContactDr Aseefhali Bankapur
Manipal Institute of Applied Physics, MAHE, Manipal
Outcome results
None listed