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Newer techniques in diagnosis of leprosy

Diagnostic efficacy of Auramine-O staining in slit skin smear versus polymerase chain reaction in skin biopsy sample among clinically diagnosed patients of leprosy- a hospital based cross- sectional study - NIL

Status
Active, not recruiting
Phases
Unknown
Study type
Interventional
Source
CTRI
Registry ID
CTRI/2025/03/083431
Enrollment
87
Registered
2025-03-25
Start date
Unknown
Completion date
Unknown
Last updated
2025-04-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Health Condition 1: A30- Leprosy [Hansens disease]

Interventions

Intervention1: polymerase chain reaction: polymerase chain reaction of a skin biopsy is done which has good sensitivity and specificity Intervention2: Auramine-O: slit skin smear sample for ear lobe

Sponsors

Shri Ram Murti Smarak Institue of Medical Sciences
Lead Sponsor

Eligibility

Inclusion criteria

Inclusion criteria: Clinically diagnosed patients of leprosy as per the consensus of two qualified dermatologists working in the department.

Exclusion criteria

Exclusion criteria: Patient/ guardians of patients unwilling to give written informed consent with photography. Hypersensitivity to local anesthesia.

Design outcomes

Primary

MeasureTime frame
In a study by Girma S, Avanzi C, Bobosha K, Desta K, Idriss MH, Busso P, et al, Auramine-O was considered a user friendly method and good for peripheral centers where trained staff is available with difficulty. It also showed that Auramine-O has better sensitivity than ZN staining as it was easier to read. Whereas PCR was considered good for urban centers where there is no shortage of resources in terms of money and trained manpower.Timepoint: baseline

Secondary

MeasureTime frame
result of Auramine-O obtained in 1 day can help in starting the treatment earlyTimepoint: 1 day

Countries

India

Contacts

Public ContactTanya Gupta

Shri Ram Murti Smarak Institute of Medical Sciences

drpratikg@rediffmail.com9319702210

Outcome results

None listed

Source: CTRI (via WHO ICTRP) · Data processed: Feb 4, 2026