None listed
Conditions
Interventions
Intervention1: Nil: Nil
Control Intervention1: Clear aligner therapy: Saliva of Participants who are willing to undergo clear aligner therapy, will be assessed for Microplastics(FTIR) and their effect
Sponsors
AB Shetty Memorial Institute of Dental Sciences
Eligibility
Inclusion criteria
Inclusion criteria: 1) Individuals opting for clear aligner therapy 2) Individuals between 18 years to 24 years of age 3) No previous history of orthodontic treatment 4) Individuals willing to participate in the study
Exclusion criteria
Exclusion criteria: 1) Individuals who use tobacco in any form 2) Individuals on medications for systemic conditions 3) Individuals under hormonal replacement therapy 4) Individuals with craniofacial syndromes 5) Individuals with poor oral hygiene 6) Individuals with poor periodontal conditions
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Microplastics will be assessed using Fourier Transform Infrared spectroscopy The sample will then be tested with:- 1) MTT Assay/ 3-(4,5- dimethylthiazol-2-yl)-2,5-Diphenyl Tetrazolium bromide assay: To evaluate the cytotoxicity of microplastics on primary cultures of human gingival fibroblasts. 2) MCF-7 cell line test: To assess estrogenicity of microplastics. 3) MPO Assay: To assess whether microplastics cause oxidative stress.Timepoint: T0 : control (before onset of treatment) T1: 1 day after onset of treatment T2 : 14 days after onset of treatment T3: 30 days after onset of treatment | — |
Secondary
| Measure | Time frame |
|---|---|
| The results of this study will emphasize the necessity of adopting sustainable practices. The study aligns with the following Sustainable Development Goals i.e. SDG 3 Good health & well being SDG12 Responsible consumption & productionTimepoint: The outcomes will be assessed at 12 months post start of study | — |
Countries
India
Contacts
Public ContactDr Reuben Bensy Thomas
AB Shetty Memorial Institute of Dental Sciences
Outcome results
None listed