Health Condition 1: 1- Obstetrics Health Condition 2: E282- Polycystic ovarian syndrome
Conditions
Interventions
Intervention1: Salivary Sample Collection and Analysis for microRNA-21 Quantification: Sample Collection:
Unstimulated whole saliva will be collected from participants.
The saliva will be preserved w
Sponsors
Central Research laboratory
Eligibility
Inclusion criteria
Inclusion criteria: Volunteering participants Age criteria: 18 to 30 years Patients who are diagnosed with PCOS Gender: Females
Exclusion criteria
Exclusion criteria: Patients under treatment for PCOS Controls included any known medical conditions or disease Pregnancy and lactating women
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To evaluate the differential expression of salivary microRNA-21 in Polycystic Ovarian Syndrome (PCOS) patients compared to healthy controls, establishing its potential as a non-invasive diagnostic biomarker.Timepoint: Baseline (1–6 Months):Collect unstimulated saliva samples from PCOS patients and controls. 7th Month: Week 1: Extract microRNA. Week 2: Quantify and qualify microRNA. Week 3: Prepare microRNA-21 and RNU6 primers. Week 4: Convert RNA to cDNA. 8th Month: Week 1: Perform RT-qPCR for microRNA-21 expression. Weeks 2 and 3: Conduct statistical and ROC curve analysis to evaluate diagnostic potential. | — |
Secondary
| Measure | Time frame |
|---|---|
| nilTimepoint: nil | — |
Countries
India
Contacts
Public ContactM ABIRAMI
Karpaga Vinayaga Institute of Dental Sciences
Outcome results
None listed