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Simplified Method for Freezing Small Numbers of Human Spermatozoa using Sole Trehalose

Cryopreservation of Single Human Spermatozoa using Sole Trehalose - NIL

Status
Unknown
Phases
Unknown
Study type
Interventional
Source
CTRI
Registry ID
CTRI/2024/02/062755
Enrollment
2500
Registered
2024-02-16
Start date
Unknown
Completion date
Unknown
Last updated
2024-03-04

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Interventions

Intervention1: Trehalose based cryoprotective medium: In house Trehalose based cryoprotective medium concentration of 50 mM, 100 mM, 150 mM and 200 mM Control Intervention1: SpermFreeze (Fertipro, Bel

Sponsors

Faculty of Medicine Research Scholarship, Prince of Songkla University
Lead Sponsor

Eligibility

Inclusion criteria

Inclusion criteria: 1.Healthy male, semen concentration of 20 million per mL or more and at least 50 percent motility 2.Correct collection method

Exclusion criteria

Exclusion criteria: 1.Incorrect method : intercourse and condom used, spermicidal lubricant used 2.Abnormal semen analysis 3.Semen delivered to the laboratory for more than 1 hour

Design outcomes

Primary

MeasureTime frame
Post thaw recovery rate, post thaw motility rateTimepoint: 24 hours—

Secondary

MeasureTime frame
Post thaw progressive motility rateTimepoint: 24 hours—

Countries

Thailand

Contacts

Public ContactKriengsak Dhanaworavibul

Prince of Songkla University

wannakosaroch@gmail.com66834088099

Outcome results

None listed

Source: CTRI (via WHO ICTRP) · Data processed: Feb 4, 2026