Health Condition 1: U072- COVID 19 virus not identified
Conditions
Interventions
Intervention1: Pre- freezing vs post freezing: Technique of cryopreservation of ovarian cortical tissue
Intervention2: Technique of Cryopreservation: This is a technique for preservation of ovarian ti
Sponsors
Indian Council of Medical Research
Eligibility
Inclusion criteria
Inclusion criteria: i) Patients with good prognosis for survival after chemo/radiotherapy requiring OTC a. Pubertal ages with malignancy b. Adults in reproductive years for immediate chemo/radiotherapy with little time for oocyte/embryo freezing as this requires 2-3 weeks of ovarian stimulation and oocyte recovery and embryo culture ii) Willing to participate in the study
Exclusion criteria
Exclusion criteria: i) Patients with poor prognosis for survival ii) Medical complications not permitting laparoscopy/anaesthesia to harvest ovarian tissue iii) Not willing to participate in study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Comparing the two methods of cryopreservation we shall assess the survival of cryo- preserved ovarian tissue by assessing: ? Primordial follicle count ? Proportion of viable primordial follicle ? Extent of DNA damage to primordial folliclesTimepoint: All tissue samples will be assessed at baseline(control) and post thawing (6 months) | — |
Secondary
| Measure | Time frame |
|---|---|
| i) Proportion of intact or viable primordial follicles, ii) Primordial follicle density and DNA fragmentation in primordial follicles. iii) The primordial follicle density and intactness will be checked at light microscopy and electron microscopy (TEM). Timepoint: 01 year 02 year 03 year | — |
Countries
India
Contacts
Public ContactDr Neena Malhotra
All India Institute of Medical Sciences
Outcome results
None listed