Health Condition 1: L088- Other specified local infections of the skin and subcutaneous tissue
Conditions
Interventions
None listed
Sponsors
IADVL research grant
Eligibility
Inclusion criteria
Inclusion criteria: a) Leprosy patients with plantar ulcers who are not on topical or systemic antibiotics since atleast 2 weeks b) Age above 18 years
Exclusion criteria
Exclusion criteria: a. patients with a history of use of topical or systemic antibiotics within 2 weeks. b. Patients with ulcer who are admitted in hospital since atleast 5 days which can alter bacterial colonization
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Quantitative measurement of biofilm production by the Microtitre plate method (MTP) will be performed for in clinical isolates of bacteria. An optical density (OD) greater than 0.240 indicated a strong biofilm producer, an OD between 0.120â??0.240 a moderate biofilm producer and an OD of less than 0.120, a non-biofilm producer. S. aureus ATCC 25923 will be used as the control organism for biofilm production Timepoint: 3 days | — |
Secondary
| Measure | Time frame |
|---|---|
| The antimicrobial susceptibility testing (AST) determined by the Kirby-Bauer disc diffusion method after it is incubated for 18â??24 h at 37 °C aerobically, the isolates will be classified as susceptible, intermediate and resistant as per standard guidelines.Timepoint: 24hrs | — |
Countries
India
Contacts
Public ContactDr Michelle Serene Fernandes
Father Muller Medical College
Outcome results
None listed