Health Condition 1: K135- Oral submucous fibrosis
Conditions
Interventions
Intervention1: Epigallocatechin 3-gallate: The buccal tissue samples will be collected from patients by using biopsy technique and processed for the generation of primary cell culture. The generated p
Sponsors
Manipal College of Pharmaceutical Sciences MAHE Manipal
Dr Shruthi Acharya
Dr Usha Yogendra Nayak
Eligibility
Inclusion criteria
Inclusion criteria: Tissues can be included or taken from the patients who confirmed with oral submucous fibrosis (OSMF, disease control) disease and patients without OSMF (normal control). Also, Fibrous bands along the buccal mucosa on clinical examination; histopathological confirmation of punch biopsy.
Exclusion criteria
Exclusion criteria: Patients confirmed with oral cancer.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| As the OSMF condition increasing day by day, effective treatment strategies are required. In this study, the efficacy of the drug and formulation will be checked on the cells harvested from the biopsy tissue sample which can be used for checking efficacy and cytotoxicity.Timepoint: The tissue will be collected from the patients using biopsy technique and same will be used for the harvesting of cells from collected biopsy tissue sample which requires minimum of 2 to 3 weeks. | — |
Secondary
| Measure | Time frame |
|---|---|
| The generated cells will be used for testing the efficacy of selected polyphenols and prepared formulation of loaded polyphenol by performing the cytotoxicity studies and gene expression study. The cells will be incubated for 72 hrs. for cytotoxicity and gene expression studies. Once the incubation period finished, the cells will used for analyzing the cytotoxicity and expression of genes.Timepoint: The efficacy of the polyphenols and formulation on cells harvested from the biopsy tissue sample and same has been used for the gene expression studies which requires incubation period for almost 72 hrs (3 days). | — |
Countries
India
Contacts
Public ContactDr Shruthi Acharya
Manipal College of Dental Sciences, Manipal
Outcome results
None listed