To check the effect of sperm freezing and warming for 3 times and there by studying the amount of healthy sperm recovered.

Impact of repeat freeze-thaw on functional and genetic integrity of sperm:comparison between vitrification and rapid freezing

Status

Active, not recruiting

Phases

Phase 1

Study type

Observational

Source

CTRI

Registry ID

CTRI/2019/05/019196

Enrollment

Registered

2019-05-16

Start date

Unknown

Completion date

Unknown

Last updated

2021-11-24

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Health Condition 1: N461- Oligospermia

Interventions

Intervention1: Cryopreservation of sperm using Rapid freezing and Vitrification: storing sperm samples at subzero condition at -196C in liquid nitrogen for future use Control Intervention1: Cryopreser

Eligibility

Inclusion criteria

Inclusion criteria: 1. Patients diagnosed with Normozoospermic sample and Oligozoospermic sample according to WHO 2010 critiria are considered

Exclusion criteria

Exclusion criteria: 1. Patients diagnosed with STDs are excluded 2. Home collection samples are excluded

Design outcomes

Primary

Measure	Time frame
Optimization of sperm vitrificationTimepoint: 2 months	—

Secondary

Measure	Time frame
Viable, motile and morphological normal sperm after repeated freeze-thawTimepoint: 4 months	—

Countries

India

Contacts

Public ContactRahul

Manipal Academy of Higher Education

shweta.hegde@manipal.edu9110883847

Outcome results

None listed

Source: CTRI (via WHO ICTRP) · Data processed: Feb 4, 2026