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Comparison of Mouse Gingival Fibroblast Attachment on Periodontally involved Human Root Surfaces after Mechanical Debridement Alone or Followed by Treatment with either Citric acid, Tetracycline hydrochloride or Nd: YAG irradiation through SEM analysis and MTT Assay .

Comparative Evaluation of Murine Gingival Fibroblast Attachment on Periodontally involved Human Root Surfaces after Mechanical Debridement Alone or Followed by Treatment with either Citric acid, Tetracycline hydrochloride or Nd: YAG irradiation: An Invitro SEM and MTT Assay Study

Status
Active, not recruiting
Phases
Unknown
Study type
Interventional
Source
CTRI
Registry ID
CTRI/2018/12/016545
Enrollment
24
Registered
2018-12-06
Start date
Unknown
Completion date
Unknown
Last updated
2021-11-24

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Interventions

Intervention1: Mechanical root debridement group: In vitro Mechanical root debridement of root specimens and cultured with murine gingival fibroblasts Control Intervention1: Citric Acid group: In vitr

Sponsors

Dr Ashwini Samaleti
Lead Sponsor

Eligibility

Inclusion criteria

Inclusion criteria: 1.Periodontally involved single rooted teeth with loss of attachment more than 5mm with hopeless prognosis indicated for extraction 2.Subjects who has not undergone any non-surgical / surgical periodontal therapy 3.Systemically healthy subjects aged above 18 years

Exclusion criteria

Exclusion criteria: 1.Teeth with root caries. 2.Teeth with prosthetic restoration. 3.Teeth with restoration on root surface. 4.Teeth with endodontic treatment. 5.Teeth with fracture. 6.Teeth with attrition. 7.Teeth with abrasion. 8.Teeth with erosion. 9.Teeth with abfraction. 10.Teeth with internal or external resorption. 11.Teeth with developmental anomalies. 12.Teeth having received vital / non-vital bleaching

Design outcomes

Primary

MeasureTime frame
Scanning Electron Microscopic analysis at 1500x magnification for murine gingival fibroblast adhesion on treated root surfacesTimepoint: Assessment post culture at 96 hrs.—

Secondary

MeasureTime frame
MTT assay for murine gingival fibroblast viability on treated root surfacesTimepoint: Assessment post culture at 24 hrs, 42 hrs, 72 hrs,96 hrs.—

Countries

India

Contacts

Public ContactDr Vineet Kini

MGM DENTAL COLLEGE AND HOSPITAL, NAVI MUMBAI

drvinkin@gmail.com9769804390

Outcome results

None listed

Source: CTRI (via WHO ICTRP) · Data processed: Feb 4, 2026