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Comparison of quantity of DNA using various methods

To compare the quantity of DNA obtained from GCF and saliva samples using micropipettes, paper strips and paper points.

Status
Recruiting
Phases
Unknown
Study type
Observational
Source
CTRI
Registry ID
CTRI/2018/04/013284
Enrollment
200
Registered
2018-04-16
Start date
Unknown
Completion date
Unknown
Last updated
2021-11-24

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Health Condition 1: null- CHRONIC PERIODONTITIS

Interventions

None listed

Sponsors

DR SHIVAPRASAD BM
Lead Sponsor
DR ANJU CECIL
Collaborator

Eligibility

Inclusion criteria

Inclusion criteria: 1. All subjects willing to participate in the study. 2. Chronic periodontitis patients with 5 or more teeth with probing depth >=5mm and clinical attachment level >= 3mm and gingival index >=2 will be included . 3. All the patients should be systemically healthy and should not have received periodontal treatment for at least 6 months prior to the clinical examination and sampling.

Exclusion criteria

Exclusion criteria: Smokers. Systemic diseases like diabetes mellitus, heart diseases ,rheumatoid arthritis, hepatitis and other systemic diseases that can alter the course of periodontal disease. Subjects on any medication taken within the last 6 months which may alter the periodontal status. Pregnant and lactating mothers. Any periodontal therapy in the last 6 months

Design outcomes

Primary

MeasureTime frame
Quantity of DNA obtained from Saliva and GCF,extracted using micropipettes,paper strips and paper pointsTimepoint: Quantity of DNA obtained from Saliva and GCF,extracted using micropipettes,paper strips and paper points were assessed immediately and after 1 and 3 months respectively

Secondary

MeasureTime frame
Quantity of DNA obtained from Saliva and GCF, extracted using micropippetes, paper strips and paper pointsTimepoint: Quantity of DNA obtained from Saliva and GCF , extracted using micropippetes, paper strips and paper points were assessed immediately and after 1 and 3 months respectively

Countries

India

Contacts

Public ContactDR SHIVAPRASAD BM

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES

anju.cecil@gmail.com9916380097

Outcome results

None listed

Source: CTRI (via WHO ICTRP) · Data processed: Feb 4, 2026