To evaluate whether rapid freezing is a better method of preserving sperms compared to conventional slow freeze in male factor infertility.

To compare the efficacy of vitrification versus slow freeze as a method of cryopreservation of spermatozoa in male factor infertility. - Nil

Status

Active, not recruiting

Phases

Phase 3

Study type

Interventional

Source

CTRI

Registry ID

CTRI/2015/01/005391

Enrollment

Registered

2015-01-13

Start date

Unknown

Completion date

Unknown

Last updated

2021-11-24

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Health Condition 1: null- Washed semen samples of male infertile patients

Interventions

Intervention1: VITRIFICATION ARM: 4Î¼l sperm suspension diluted 1:1 with 0.5 M sucrose at room temperature for 2min 275Î¼l stripper tips (MEA tested) with a silicone seal -are carriers for cooling

Eligibility

Inclusion criteria

Inclusion criteria: semen samples of subfertile male patients with 1. Severe oligo astheno teratozoospermia with fresh concentration 1- 10 million, progressive motility 10 - 30% and Kruger morphology 2. Very severe oligo astheno teratozoospermia with fresh concentration <1 million, progressive motility <10%

Exclusion criteria

Exclusion criteria: semen samples of male patients with Normozoospermia with fresh concentration more than 20 million, progressive motility more than 50% and Kruger morphology more than 14%

Design outcomes

Primary

Measure	Time frame
Progressive Motility at different intervals of freezingTimepoint: 1month and 6 months	—

Secondary

Measure	Time frame
Vitality of spermatozoa will be determined by assessing the membrane integrity of the sperm after staining with Eosin â?? Nigrosin. Hyaluronan binding assay for DNA integrity Timepoint: 1 months and 6 months	—

Countries

India

Contacts

Public ContactDr Darshana Arakkal

CHRISTIAN MEDICAL COLLEGE

mmuutthhuu@yahoo.com04162283301

Outcome results

None listed

Source: CTRI (via WHO ICTRP) · Data processed: Feb 4, 2026