Influenza, Immunity against influenza
Conditions
Brief summary
Humoral immunity to influenza virus strains included in the vaccines of the current and past seasons as well as circulating influenza strains will be assessed from the serum samples with traditional hemagglutination inhibition (HI). Microneutralization or neutralization test (NT) may also be used.
Detailed description
Cellular immunity will be assessed from thawed PBMC samples after stimulation with circulating influenza virus and vaccine antigens in cell culture., Cytokine (IFN-gamma and others) responses will be assessed e.g. by measuring the concentration of secreted cytokines in the cell culture media with Luminex technology., mRNA expression of cytokines and other factors (e.g. inflammatory mediators and transcription factors) can be measured from specific cell populations enriched with magnetic cell separation or sorted with FACSAria flow cytometer.
Interventions
Sponsors
Finnish Institute For Health And Welfare
Eligibility
Sex/Gender
All
Age
18 Years to 64 Years
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Humoral immunity to influenza virus strains included in the vaccines of the current and past seasons as well as circulating influenza strains will be assessed from the serum samples with traditional hemagglutination inhibition (HI). Microneutralization or neutralization test (NT) may also be used. | — |
Secondary
| Measure | Time frame |
|---|---|
| Cellular immunity will be assessed from thawed PBMC samples after stimulation with circulating influenza virus and vaccine antigens in cell culture., Cytokine (IFN-gamma and others) responses will be assessed e.g. by measuring the concentration of secreted cytokines in the cell culture media with Luminex technology., mRNA expression of cytokines and other factors (e.g. inflammatory mediators and transcription factors) can be measured from specific cell populations enriched with magnetic cell separation or sorted with FACSAria flow cytometer. | — |
Countries
Finland
Outcome results
None listed