Rheumatoid arthritis, Systemic lupus erythematosus, Systemic sclerosis
Conditions
Brief summary
the frequency and phenotype of antigen-specific, autoreactive B cells in the peripheral blood of patients at the indicated time-points, compared to the frequency and phenotype of TT-specific B cells prior to and upon booster vaccination, at the same timepoints. Assessment will be done by flow cytometry, generating a phenotypic profile rather than a single parameter.
Detailed description
plasma levels and characteristics (e.g. glycosylation) of autoantibodies and anti-TT antibodies during different stages of activation of the underlying B cell responses., phenotype and dynamics of TT-specific B cells before and upon vaccination in healthy individuals.
Interventions
DRUGTetanusvaccin
DRUGsuspensie voor injectie 40 IE
Sponsors
Leids Universitair Medisch Centrum (LUMC)
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| the frequency and phenotype of antigen-specific, autoreactive B cells in the peripheral blood of patients at the indicated time-points, compared to the frequency and phenotype of TT-specific B cells prior to and upon booster vaccination, at the same timepoints. Assessment will be done by flow cytometry, generating a phenotypic profile rather than a single parameter. | — |
Secondary
| Measure | Time frame |
|---|---|
| plasma levels and characteristics (e.g. glycosylation) of autoantibodies and anti-TT antibodies during different stages of activation of the underlying B cell responses., phenotype and dynamics of TT-specific B cells before and upon vaccination in healthy individuals. | — |
Countries
Netherlands
Outcome results
None listed